FIGURE
Fig. 4
- ID
- ZDB-FIG-061227-33
- Publication
- Kurita et al., 2004 - Transgenic zebrafish produced by retroviral infection of in vitro-cultured sperm
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Fig. 4
Analysis of transmission of the retroviral insertion into progenies by PCR and Southern blot hybridization. (A) PCR analysis with DNA extracted from progeny of each positive founder fish in experiments 3 and 5. Almost half of the progeny are positive for the provirus, indicating the transgene is not mosaic in the founder fish. (B) Southern blot analysis of genomic DNA from a positive founder fish and four individual PCR-positive progeny. Genomic DNA samples were digested with BglII. All PCR-positive progenies show the identical integration site to the parent. |
Expression Data
Expression Detail
Antibody Labeling
Phenotype Data
Phenotype Detail
Acknowledgments
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Full text @ Proc. Natl. Acad. Sci. USA