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Fig. 4 Analysis of transmission of the retroviral insertion into progenies by PCR and Southern blot hybridization. (A) PCR analysis with DNA extracted from progeny of each positive founder fish in experiments 3 and 5. Almost half of the progeny are positive for the provirus, indicating the transgene is not mosaic in the founder fish. (B) Southern blot analysis of genomic DNA from a positive founder fish and four individual PCR-positive progeny. Genomic DNA samples were digested with BglII. All PCR-positive progenies show the identical integration site to the parent.
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Full text @ Proc. Natl. Acad. Sci. USA