FIGURE

Fig. 2

ID

ZDB-FIG-061227-31

Publication

Kurita et al., 2004 - Transgenic zebrafish produced by retroviral infection of in vitro-cultured sperm

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Fig. 2

Detection of transgenesis in fish created from infected in vitro sperm cultures. (A) PCR analysis using DNA extracted from individual founder fish. Two pairs of primers were used for PCR analysis of genomic DNA, one specific to the proviral DNA and a second specific to the zebrafish Wnt5a. The numbered lanes show the PCR products generated with DNA from individuals in a part of each experiment of Table 1 to screen the positive. Lane PC shows the positive control PCR products generated with DNA from a zebrafish cell line that contains a proviral insertion. (B) Photos of transgenic zebrafish from infected in vitro sperm cultures. The WT cultured sperm were used to fertilize extruded eggs of the albino type.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA