FIGURE

Fig. 2

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ZDB-FIG-061130-2
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Ochi et al., 2006 - Hhip regulates zebrafish muscle development by both sequestering Hedgehog and modulating localization of Smoothened
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Fig. 2

Cooperative activity of Hhip and Patched is required for restricted expression of myod in adaxial cells. (A–B) ptc1 is expressed in adaxial cells at bud stage in a control embryo (A) and is reduced by overexpression of hhip (B, arrow; 17/28 injected embryos). (C–F) In contrast, overexpression of hhip does not affect expression of wnt11, a marker of notochord, (C–D; 9/9 injected embryos) or dmrt2, a marker of paraxial mesoderm and somites (E–F, 13/14 injected embryos). (G–H) Overexpression of hhip inhibits myod expression. (G) myod is detected in adaxial cells and somites in a control embryo at the 8-somite stage. (H) myod expression is strongly reduced in a hhip mRNA-injected embryo (arrow, 25/46 injected embryos). (I–K) ptc1 expression is increased in a hhip-MO-injected embryo (I, arrow; 7/9 injected embryos). hhip-MO does not affect expression of wnt11 (J, 5/6 injected embryos) or dmrt2 (K, 11/11 injected embryos). (L–O) Reduction of Hhip and Ptc results in spread of myod expression laterally in the paraxial mesoderm. (L) Expression of myod in a control embryo. (M) Expression of myod is slightly upregulated in cells adjacent to adaxial cells in a hhip-MO-injected embryo (arrow, 23/93 injected embryos). (N) Injection of ptc-MO has a stronger effect than injection of hhip-MO (arrow, 21/27 injected embryos). (O) Reduction of both Hhip and Ptc causes a significant increase in myod expression in paraxial mesoderm (arrow, 27/27 injected embryos). Dorsal views, bud stage (A–D, I–J, L–O) and 8-somite stage (E–H, K), anterior towards the top. Scale bar: 200 μm.

Expression Data
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Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: Bud to 5-9 somites

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
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Reprinted from Developmental Biology, 297(1), Ochi, H., Pearson, B.J., Chuang, P.T., Hammerschmidt, M., and Westerfield, M., Hhip regulates zebrafish muscle development by both sequestering Hedgehog and modulating localization of Smoothened, 127-140, Copyright (2006) with permission from Elsevier. Full text @ Dev. Biol.