FIGURE

Fig. 7

ID
ZDB-FIG-070920-23
Publication
Ochi et al., 2006 - Hhip regulates zebrafish muscle development by both sequestering Hedgehog and modulating localization of Smoothened
Other Figures
All Figure Page
Back to All Figure Page
Fig. 7

Hhip regulates Smoothened localization. (A–D) Smo is detected intracellularly (B, asterisk) and at the cell surface (B, arrow and broken line) in cells near the notochord (A, B, D, 93%, n = 16) compared with lateral cells that contain primarily intracellular Smo (C, 50%, n = 6). (E, F, G, H) hhip-MO suppresses internalization of Smo in medial cells (83%, n = 18). (I, J, K, L) Inhibition of endocytosis suppresses internalization of Smo (100%, n = 7). 100 μM MDC added at the 40% epiboly stage and then incubated to the 8-somite stage. (A–L) Embryos were injected with myc-tagged smo plasmid at the 1-cell stage and incubated to the 8-somite stage. Single confocal images of myc-tagged Smo (A–G, I, J, K and D, H, L, upper panels). Embryos were labeled with anti-myc antibody and myod (A, E, I) or anti-β-catenin antibody (D, H, L), a marker of the membrane (Nagafuchi, 2001). (D, H, L, Lower panels) Confocal micrographs (transverse z series) through myc-tagged Smo expressing cells. (A, E, I) Arrows indicate the cells shown at higher magnification in right panels. (B, C, D, F, G, H, J, K) Asterisks and arrows indicate intracellular and surface localization of Smo, respectively. N, notochord, scale bar: (A, E, I) 10 μm, (B, C, D, F, G, H, J, K, L) 2.5 μm.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Knockdown Reagents:
Observed In:
Stage: Prim-5

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 297(1), Ochi, H., Pearson, B.J., Chuang, P.T., Hammerschmidt, M., and Westerfield, M., Hhip regulates zebrafish muscle development by both sequestering Hedgehog and modulating localization of Smoothened, 127-140, Copyright (2006) with permission from Elsevier. Full text @ Dev. Biol.