FIGURE

Fig. 2

ID

ZDB-FIG-060726-4

Publication

Brand et al., 1996 - Mutations in zebrafish genes affecting the formation of the boundary between midbrain and hindbrain

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Fig. 2

Apoptosis in the tectum of noi mutant embryos. Shown is a comparison of Nomarski (A-C) and fluourescence (D-F) images of the same embryos, stained with acridine orange to detect apoptotic cell death. (A,D) Wild-type embryo at 22 h of development; (B,E) homozygous mutant noi^tu29a embryo at 22 h, stained with acridine orange. (C,F) Homozygous mutant noi^ty22b embryo at 26 h, stained with acridine orange. Large numbers of stained cells are observed in the tectum of mutant embryos in E and F (arrows) that are absent in the wild type. Degenerating, more refractile cells are also seen with Nomarski optics in C (between arrowheads). Scale bar, 120 µm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	pax2a^tu29a/tu29a pax2a^ty22b/ty22b
Observed In:	optic tectum optic tectum cell
Stage Range:	26+ somites to Prim-5

Phenotype Detail

Acknowledgments

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