FIGURE

Fig. 4

ID

ZDB-FIG-060623-13

Publication

Blasiole et al., 2006 - Separate Na,K-ATPase genes are required for otolith formation and semicircular canal development in zebrafish

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Fig. 4

Expression of semicircular canal markers in α1a.2 morphant ears. All panels are lateral views with anterior to the left. Embryos were injected with 2 ng of α1a.2 MO-1 at the one-cell stage. (A–D) ncs-1a staining in the otic vesicle (OV) of (A) 48 hpf wild type (WT) embryo, (B) 48 hpf morphant, (C) 72 hpf WT embryo, and (D) 72 hpf morphant. (E–H) dfna5 staining in OV of (E) 48 hpf WT embryo, (F) 48 hpf morphant, (G) 72 hpf WT embryo, and (H) 72 hpf morphant. (I–L) ugdh staining in OV of (I) 48 hpf WT embryo, (J) 48 hpf morphant, (K) 72 hpf WT embryo, and (L) 72 hpf morphant. mo, morphant. Scale bar: 25 μm.

Expression Data

Genes:	gsdmeb ncs1a ugdh
Fish:	WT WT + MO1-atp1a1a.2
Knockdown Reagent:	MO1-atp1a1a.2
Anatomical Terms:	otic vesicle semicircular canal
Stage Range:	Long-pec to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 294(1), Blasiole, B., Canfield, V.A., Vollrath, M.A., Huss, D., Mohideen, M.A., Dickman, J.D., Cheng, K.C., Fekete, D.M., and Levenson, R., Separate Na,K-ATPase genes are required for otolith formation and semicircular canal development in zebrafish, 148-160, Copyright (2006) with permission from Elsevier. Full text @ Dev. Biol.