FIGURE

Fig. 3

ID

ZDB-FIG-070917-126

Publication

Blasiole et al., 2006 - Separate Na,K-ATPase genes are required for otolith formation and semicircular canal development in zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Fig. 3

Knockdown of Na,K-ATPase α1a.2 mRNA disrupts development of semicircular canals. All panels show a lateral view with anterior to the left. Morphants were injected with 2 ng of α1a.2 MO-1. (A) Wild type (WT) embryo at 45 hpf. (B) α1a.2 morphant at 45 hpf. (C) Otic vesicle (OV) of WT embryo at 55 hpf. Arrows indicate protrusions of semicircular canals. (D) OV of α1a.2 morphant at 55 hpf. (E) OV of WT embryo at 5 dpf. Double-headed arrows show hubs of the semicircular canals. Arrowheads indicate otoliths. (F) OV of α1a.2 morphant at 5 dpf. Asterisk shows epithelial mass in center of OV. Arrowheads indicate otoliths. Arrow indicates the posterior crista. (G) Effect of MOs on translation of α1a.2 mRNA. α1a.2 mRNA was translated in the presence of antisense MOs (4 μM) and analyzed as described in Fig. 1A. mo, morphant. Scale bars: A–B, 250 μM; C–F, 50 μM.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 294(1), Blasiole, B., Canfield, V.A., Vollrath, M.A., Huss, D., Mohideen, M.A., Dickman, J.D., Cheng, K.C., Fekete, D.M., and Levenson, R., Separate Na,K-ATPase genes are required for otolith formation and semicircular canal development in zebrafish, 148-160, Copyright (2006) with permission from Elsevier. Full text @ Dev. Biol.