ZFIN ID: ZDB-FIG-060125-5
Croushore et al., 2005 - Ptena and ptenb genes play distinct roles in zebrafish embryogenesis. Developmental dynamics : an official publication of the American Association of Anatomists   234(4):911-921 Full text @ Dev. Dyn.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage Range: Shield to Adult

Fig. 3 Expression of zebrafish ptenb mRNA during embryogenesis. Expression of ptenb was analyzed by RT-PCR and whole-mount in situ hybridization. A: Expression of ptenb splice variants during zebrafish development determined by RT-PCR. Bands of 298 and 229 bp represent fragments of the long and short splice variants, respectively. B-K: Expression of ptenb determined by whole-mount in situ hybridization. B: Early somitogenesis, lateral view. C-H: Mid-somitogenesis. C: Ten-somite stage, lateral view. D: 10-somite stage, dorsal view of somites obtained with differential interference contrast microscopy. E: Ten-somite stage, transverse section. F: Eighteen-somite stage, lateral view. G: Eighteen-somite stage, dorsal view of tail. H: Eighteen-somite stage, dorsal view of head. I: Twenty-four hpf, lateral view. J: Twenty-four hpf, lateral view of head. K: Thirty-six hpf, lateral view of otic vesicle. L: Forty-eight hpf, lateral view. M: Five dpf, lateral view. AC, adaxial cells; BA, branchial arches; CG, cranial ganglia; D, diencephalon; EPI, epiphysis; E, eye; NC, notochord; OV, otic vesicle; PF, pectoral fin; S, somite; SC, spinal cord; TEL, telencephalon; TEG, tegmentum.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
ptenb WT standard conditions Shield to Adult whole organism RTPCR
1-4 somites to 5-9 somites somite ISH
10-13 somites adaxial cell ISH
10-13 somites somite ISH
14-19 somites cranial ganglion ISH
14-19 somites diencephalon ISH
14-19 somites epiphysis ISH
14-19 somites neural rod ISH
14-19 somites somite ISH
Prim-5 adaxial cell ISH
Prim-5 cranial ganglion ISH
Prim-5 somite ISH
Prim-5 spinal cord ISH
Prim-25 unspecified ISH
Long-pec eye ISH
Long-pec pectoral fin ISH
Long-pec pharyngeal arch ISH
Day 5 central nervous system ISH
Day 5 eye ISH
Day 5 pectoral fin ISH
Day 5 pharyngeal arch ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dev. Dyn.