Fig. 8

Right-side overexpression of XCR2 causes left-right inverted gene expression. (A-D,G-I,M-O) In situ hybridization of XNR1 in dorsal view (A-D,G,I,M,O), anterior side at top, and in side view of a half-dissected embryo (H,N). (E,F,J,K,P,Q) In situ hybridization of XATV in ventral view (E,F) and in dorsal view (J-K,P,Q). (L,R) In situ hybridization of XCR2 in dorsal view. (A,G-H) Embryo injected with 40 pg of pCS-EGFP plasmid in the right side. (B,M-R) Embryo injected with 40 pg of pCS-XCR2 into the right side. (A,E) Embryo showing normal expression of XNR1 (A) or XATV (E) in the left LPM (arrow) at stage 25/26. (B,F) Right-side XCR2 overexpression caused right-side expression of XNR1 (B) or XATV (F; arrow), and suppressed the left-side expression at stage 25/26. (C,D) Injection of 40 pg of pCS-XCR2 mEGF (C) or pCS-XCR2 mCFC (D) into the right side did not affect the expression pattern of XNR1 (arrow) at stage 25. (G-L) Embryos injected with pCS-EGFP plasmid in the right side. XNR1 expression was not detected in the LPM at stage 15 (G) and stage 16 (H), but was detectable at stage 20 (I) (arrow). XATV expression was not detected in the LPM at stage 17 (J), but was detectable at stage 23 (K; arrow). XCR2 was expressed broadly at stage 20 (L). (M-R) Embryo injected with pCS-XCR2 plasmid in the right side. XNR1 expression was ectopically expressed in the right LPM at stage 15 (M) and stage 16 (N), in addition to posterior bilateral expression. Strong XNR1 expression was detected on the right side (arrow), but not on the left side at stage 20 (O). Ectopic XATV expression (arrow) was detected in the right LPM at stage 17 (P). XATV expression was detected on the right side (arrow), but not on the left side at stage 23 (Q). Left-side expression of XCR2 was not affected, but very strong expression was detected on the right (injected) side at stage 20 (R).