ZFIN ID: ZDB-FIG-050609-6
Schäfer et al., 2005 - Medial floor plate formation in zebrafish consists of two phases and requires trunk-derived Midkine-a. Genes & Development   19(8):897-902 Full text @ Genes & Dev.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: 10-13 somites

Fig. 5 mdka transcription is not regulated by early acting floor plate-inducing factors. (a,b) In mib-/- embryos, identified by an enlarged notochord and an altered somite shape, mdka expression is normal compared with wild-type embryos (n = 14). (c,e,g) ntl, oep, and flh are expressed in the notochord of the trunk and progenitor cells in the tailbud. (c) In addition, ntl is also expressed in the posterior tailbud region. (d) In ntl-/- mutants that lack a notochord and have an enlarged MFP, mdka expression is not impaired (n = 24). In these embryos, the paraxial mesoderm is separated at the midline by an expanded MFP. (f) oep-/- mutants identified by their characteristic head phenotype form a normal notochord, but lack MFP. In oep-/-, mdka expression is normal (n = 11). (h) In flh-/- mutants, mdka is expressed at the midline (n = 25). (i) Two-phase model for MFP formation during neurulation in the zebrafish embryo. Midline precursor cells (MPC) in the tailbud are initially induced to adopt MFP fate in a first Mdka-independent phase. As the embryo extends caudally, Mdka derived from the trunk paraxial mesoderm (pm) regulates allocation of MFP cells (second phase). All pictures are dorsal views of the posterior trunk and tail; anterior is to the top. Arrowheads mark the position of the posterior front of mdka expression in the paraxial mesoderm. Bar: h, 100 µm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
mdka WT standard conditions 10-13 somites paraxial mesoderm ISH
mib1ta52b/ta52b standard conditions 10-13 somites paraxial mesoderm ISH
noton1/n1 (AB) standard conditions 10-13 somites paraxial mesoderm ISH
tbxtab195/b195 standard conditions 10-13 somites paraxial mesoderm ISH
tdgf1m134/m134 (AB) standard conditions 10-13 somites paraxial mesoderm ISH
noto WT standard conditions 10-13 somites notochord ISH
tbxta WT standard conditions 10-13 somites notochord ISH
tdgf1 WT standard conditions 10-13 somites notochord ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Genes & Dev.