FIGURE

Fig. 3

ID

ZDB-FIG-050209-14

Publication

Tyurina et al., 2005 - Zebrafish Gli3 functions as both an activator and a repressor in Hedgehog signaling

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Fig. 3

Hh signaling negatively regulates gli3 expression. (A) In 20-h wild-type embryos, gli3 is expressed regionally in the dorsal neural tube, with strong expression in the tectum and midbrain/hindbrain boundary (arrowheads). (B) gli3 expression is expanded ventrally in smu(smo) mutant embryos that lack Hh pathway activity (arrows). (C) gli3 expression is generally reduced in shh mRNA-injected wild-type embryos (compare to lacZ mRNA-injected embryo in inset). (D) gli3MO injection also led to severely reduced gli3 expression levels (compare to control MO-injected wild-type embryo in inset). All panels show gli3 expression in 20-h zebrafish embryos. Lateral views of head, anterior to the left, eyes and yolk removed.

Expression Data

Gene:	gli3
Fish:	WT + MO1-gli3 + MO2-gli3 smo^unspecified
Knockdown Reagents:	MO1-gli3 MO2-gli3
Anatomical Term:	midbrain hindbrain boundary
Stage:	20-25 somites

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 277(2), Tyurina, O.V., Guner, B., Popova, E., Feng, J., Schier, A.F., Kohtz, J.D., and Karlstrom, R.O., Zebrafish Gli3 functions as both an activator and a repressor in Hedgehog signaling, 537-556, Copyright (2005) with permission from Elsevier. Full text @ Dev. Biol.