CRISPR

CRISPR3-ret

ID

ZDB-CRISPR-240102-4

Name

CRISPR3-ret

Previous Names

None

Target

ret (1)

Sequence

5' - GAGACAGCGAGGAGACTGTG - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was "AGG" at the 3' end.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
wmr1	ret

Expression

Gene expression in Wild Types + CRISPR3-ret

No data available

Phenotype

Phenotype resulting from CRISPR3-ret

No data available

Phenotype of all Fish created by or utilizing CRISPR3-ret

Phenotype	Fish	Conditions	Figures
migratory neural crest cell division decreased frequency, abnormal	ret^wmr1/+; em2Tg/em2Tg	ultraviolet light	Fig. 4 , Fig. 5 from Baker et al., 2022
enteric nervous system development decreased process quality, abnormal	ret^wmr1/+; em2Tg/em2Tg	ultraviolet light	Fig. 2 from Baker et al., 2022
gut migratory neural crest cell Kaede expression decreased amount, abnormal	ret^wmr1/+; em2Tg/em2Tg	ultraviolet light	Fig. 2 , Fig. 3 from Baker et al., 2022
enteric neuron pbx3b expression increased amount, abnormal	ret^wmr1/+; em2Tg/em2Tg	ultraviolet light	Fig. 6 from Baker et al., 2022
gut migratory neural crest cell decreased velocity, abnormal	ret^wmr1/+; em2Tg/em2Tg	ultraviolet light	Fig. 5 from Baker et al., 2022
enteric neuron ab1-elavl labeling increased amount, abnormal	ret^wmr1/+; em2Tg/em2Tg	ultraviolet light	Fig. 7 from Baker et al., 2022
gut neural crest cell migration decreased process quality, abnormal	ret^wmr1/+; em2Tg/em2Tg	ultraviolet light	Fig. 2 , Fig. 3 from Baker et al., 2022
gut neural crest cell differentiation temporal distribution quality, abnormal	ret^wmr1/+; em2Tg/em2Tg	ultraviolet light	Fig. 6 , Fig. 7 from Baker et al., 2022
gut migratory neural crest cell ab7-h3 labeling decreased amount, abnormal	ret^wmr1/+; em2Tg/em2Tg	ultraviolet light	Fig. 5 from Baker et al., 2022
enteric nervous system development decreased process quality, abnormal	ret^wmr1/wmr1; em2Tg/em2Tg	ultraviolet light	Fig. 2 from Baker et al., 2022
gut migratory neural crest cell Kaede expression decreased amount, abnormal	ret^wmr1/wmr1; em2Tg/em2Tg	ultraviolet light	Fig. 2 from Baker et al., 2022
gut neural crest cell migration decreased process quality, abnormal	ret^wmr1/wmr1; em2Tg/em2Tg	ultraviolet light	Fig. 2 from Baker et al., 2022

Citations