CRISPR

CRISPR6-nkx3-2

ID

ZDB-CRISPR-230406-1

Name

CRISPR6-nkx3-2

Previous Names

None

Target

nkx3-2 (1)

Sequence

5' - GATCAGGAATCCGCGGCCAA - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

None

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
uu2803	nkx3-2

Expression

Gene expression in Wild Types + CRISPR6-nkx3-2

No data available

Phenotype

Phenotype resulting from CRISPR6-nkx3-2

No data available

Phenotype of all Fish created by or utilizing CRISPR6-nkx3-2

Phenotype	Fish	Conditions	Figures
scaphium absence of anatomical entity, abnormal	nkx3-2^{uu2803/uu2803}; ba2Tg (AB)	standard conditions	Fig 8 from Waldmann et al., 2021
mouth open, abnormal	nkx3-2^{uu2803/uu2803}; ba2Tg (AB)	standard conditions	Fig 1 , Fig 5 , Fig 6 from Waldmann et al., 2021
palatoquadrate cartilage increased thickness, abnormal	nkx3-2^{uu2803/uu2803}; ba2Tg (AB)	standard conditions	Fig 4 from Waldmann et al., 2021
palatoquadrate cartilage chondrocyte circular, abnormal	nkx3-2^{uu2803/uu2803}; ba2Tg (AB)	standard conditions	Fig 2 from Waldmann et al., 2021
basioccipital fused with exoccipital, abnormal	nkx3-2^{uu2803/uu2803}; ba2Tg (AB)	standard conditions	Fig 8 from Waldmann et al., 2021
vertebra 1 absence of anatomical entity, abnormal	nkx3-2^{uu2803/uu2803}; ba2Tg (AB)	standard conditions	Fig 8 from Waldmann et al., 2021
mandibular symphysis increased thickness, abnormal	nkx3-2^{uu2803/uu2803}; ba2Tg (AB)	standard conditions	Fig 6 from Waldmann et al., 2021
mandibular symphysis deformed, abnormal	nkx3-2^{uu2803/uu2803}; ba2Tg (AB)	standard conditions	Fig 6 from Waldmann et al., 2021
tripus deformed, abnormal	nkx3-2^{uu2803/uu2803}; ba2Tg (AB)	standard conditions	Fig 8 from Waldmann et al., 2021
Meckel's cartilage-palatoquadrate cartilage joint increased mass density, abnormal	nkx3-2^{uu2803/uu2803}; ba2Tg (AB)	standard conditions	Fig 4 from Waldmann et al., 2021
palatoquadrate cartilage chondrocyte decreased size, abnormal	nkx3-2^{uu2803/uu2803}; ba2Tg (AB)	standard conditions	Fig 2 from Waldmann et al., 2021
palatoquadrate cartilage chondrocyte disorganized, abnormal	nkx3-2^{uu2803/uu2803}; ba2Tg (AB)	standard conditions	Fig 2 from Waldmann et al., 2021
Meckel's cartilage-palatoquadrate cartilage joint fused with Meckel's cartilage-palatoquadrate cartilage joint, abnormal	nkx3-2^{uu2803/uu2803}; ba2Tg (AB)	standard conditions	Fig 2 , Fig 3 , Fig 4 , Fig 5 from Waldmann et al., 2021
palatoquadrate cartilage increased intensity, abnormal	nkx3-2^{uu2803/uu2803}; ba2Tg (AB)	standard conditions	Fig 4 from Waldmann et al., 2021
Meckel's cartilage-palatoquadrate cartilage joint chondrocyte aligned with Meckel's cartilage-palatoquadrate cartilage joint chondrocyte, abnormal	nkx3-2^{uu2803/uu2803}; ba2Tg (AB)	standard conditions	Fig 3 from Waldmann et al., 2021
vertebra 2 parapophysis 2 decreased size, abnormal	nkx3-2^{uu2803/uu2803}; ba2Tg (AB)	standard conditions	Fig 8 from Waldmann et al., 2021
Meckel's cartilage-palatoquadrate cartilage joint chondrocyte hypertrophic, abnormal	nkx3-2^{uu2803/uu2803}; ba2Tg (AB)	standard conditions	Fig 3 from Waldmann et al., 2021
vertebra parapophysis absence of anatomical entity, abnormal	nkx3-2^{uu2803/uu2803}; ba2Tg (AB)	standard conditions	Fig 7 from Waldmann et al., 2021
Meckel's cartilage intensity, abnormal	nkx3-2^{uu2803/uu2803}; ba2Tg (AB)	standard conditions	Fig 4 from Waldmann et al., 2021
auditory capsule posterior side decreased length, abnormal	nkx3-2^{uu2803/uu2803}; ba2Tg (AB)	standard conditions	Fig 4 from Waldmann et al., 2021
Meckel's cartilage-palatoquadrate cartilage joint cartilage tissue increased intensity, abnormal	nkx3-2^{uu2803/uu2803}; ba2Tg (AB)	standard conditions	Fig 4 from Waldmann et al., 2021
basioccipital anterior region convex, abnormal	nkx3-2^{uu2803/uu2803}; ba2Tg (AB)	standard conditions	Fig 8 from Waldmann et al., 2021
retroarticular process absence of anatomical entity, abnormal	nkx3-2^{uu2803/uu2803}; ba2Tg (AB)	standard conditions	Fig 2 , Fig 5 from Waldmann et al., 2021
cranium morphology, abnormal	nkx3-2^{uu2803/uu2803}; ba2Tg (AB)	standard conditions	Fig 6 from Waldmann et al., 2021
exoccipital fused with exoccipital, abnormal	nkx3-2^{uu2803/uu2803}; ba2Tg (AB)	standard conditions	Fig 8 from Waldmann et al., 2021
claustrum bone absence of anatomical entity, abnormal	nkx3-2^{uu2803/uu2803}; ba2Tg (AB)	standard conditions	Fig 8 from Waldmann et al., 2021

Citations