CRISPR

CRISPR1-aldh3a1

ID

ZDB-CRISPR-210817-5

Name

CRISPR1-aldh3a1

Previous Names

None

Target

aldh3a1 (1)

Sequence

5' - GGGGTCTGGATCTGCCTGAC - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was "TGG" at the 3' end

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
zf3260	aldh3a1

Expression

Gene expression in Wild Types + CRISPR1-aldh3a1

No data available

Phenotype

Phenotype resulting from CRISPR1-aldh3a1

No data available

Phenotype of all Fish created by or utilizing CRISPR1-aldh3a1

Phenotype	Fish	Conditions	Figures
whole organism ins expression decreased amount, abnormal	aldh3a1^{zf3260/zf3260}	standard conditions	Fig. 5 from Lou et al., 2020
whole organism asparagine decreased amount, abnormal	aldh3a1^{zf3260/zf3260}	standard conditions	Fig. 7 , Fig. 8 from Lou et al., 2020
whole organism glucose increased amount, abnormal	aldh3a1^{zf3260/zf3260}	standard conditions	Fig. 7 from Lou et al., 2020
whole organism 4-hydroxynon-2-enal increased amount, abnormal	aldh3a1^{zf3260/zf3260}	standard conditions	Fig. 8 from Lou et al., 2020
whole organism cysteine decreased amount, abnormal	aldh3a1^{zf3260/zf3260}	standard conditions	Fig. 8 from Lou et al., 2020
glucose homeostasis disrupted, abnormal	aldh3a1^{zf3260/zf3260}	standard conditions	Fig. 7 from Lou et al., 2020
whole organism pdx1 expression decreased amount, abnormal	aldh3a1^{zf3260/zf3260}	standard conditions	Fig. 5 from Lou et al., 2020
whole organism methionine decreased amount, abnormal	aldh3a1^{zf3260/zf3260}	standard conditions	Fig. 7 , Fig. 8 from Lou et al., 2020
ocular blood vessel increased diameter, abnormal	aldh3a1^{zf3260/zf3260}; y1Tg	control	Fig. 4 from Lou et al., 2020
intersegmental vessel morphology, abnormal	aldh3a1^{zf3260/zf3260}; y1Tg	control	Fig. 3 from Lou et al., 2020
whole organism ATP decreased amount, abnormal	aldh3a1^{zf3260/zf3260} + MO1-pdx1	standard conditions	Fig. 7 from Lou et al., 2020
whole organism ADP decreased amount, abnormal	aldh3a1^{zf3260/zf3260} + MO1-pdx1	standard conditions	Fig. 7 from Lou et al., 2020
whole organism glucose increased amount, exacerbated	aldh3a1^{zf3260/zf3260} + MO1-pdx1	standard conditions	Fig. 7 from Lou et al., 2020
intersegmental vessel morphology, exacerbated	aldh3a1^{zf3260/zf3260}; y1Tg + MO1-pdx1	standard conditions	Fig. 3 from Lou et al., 2020
ocular blood vessel increased diameter, abnormal	aldh3a1^{zf3260/zf3260}; y1Tg + MO1-pdx1	standard conditions	Fig. 4 from Lou et al., 2020
intersegmental vessel increased branchiness, exacerbated	aldh3a1^{zf3260/zf3260}; y1Tg + MO1-pdx1	standard conditions	Fig. 3 from Lou et al., 2020
ocular blood vessel sprouting angiogenesis increased process quality, exacerbated	aldh3a1^{zf3260/zf3260}; y1Tg + MO1-pdx1	standard conditions	Fig. 4 from Lou et al., 2020

Citations