Figure 1

The variation of body index, fasting blood glucose, triglycerides, and total cholesterol in male zebrafish after overfeeding. The body length (A), body weight (B), condition factor index (C), fasting blood glucose (D), and the levels of triglycerides (E) and total cholesterol (F) in the serum of male zebrafish were significantly increased in the overfed groups (*, p < 0.05; **, p < 0.01; n = 13).
Figure 2

Accumulation of lipid droplets in livers of overfed zebrafish. Images of Oil Red O staining for section of livers in control and overfed groups (A). The fold change of lipid droplet accumulation according to the results of Oil Red O staining between the control group and the overfed group were quantified by Image J software (version 1.50i) and showed in histogram (B). The levels of triglyceride (C) and cholesterol (D) from liver in control and overfed group. (Values are means ± SEM; *, p < 0.05; **, p < 0.01; CK: control group, OF: overfed group; n = 5.)
Figure 3

The results of data analysis for the transcriptome of livers in control and overfed groups. Principal component analysis (PCA) of gene expression profiles for differentially expressed genes (A). The number of differentially expressed genes (DEGs) for up- and down-regulated in zebrafish liver after overfeeding is shown in the histogram (B). (Fold change ≥ 1.5 and p-value ≤ 0.05.)
Figure 4

The results of KEGG enrichment analysis for DEGs in livers after overfeeding. Dot plot of KEGG enrichment analysis results for up-regulated DEGs (A) and down-regulated DEGs (B). Network of the top 10 hub pathways with the highest maximal clique centrality (MCC) for up-regulated DEGs (C) and down-regulated DEGs (D). (Edges between nodes represent Jaccard similarity coefficients; the colors and sizes of nodes stand for the p-value of pathways and the number of genes in the pathway, respectively.)
Figure 5

Identified hub genes in the hub pathways. Network of the 11 hub genes mapped to top 4 hub pathways enriched from up-regulated DEGs (A). The fold change of up-regulated hub genes after overfeeding (B). Network of 14 hub genes mapped to top 5 hub pathways enriched from down-regulated DEGs (C). The fold change of down-regulated hub genes after overfeeding (D). Round and diamond stand for genes and pathways, respectively; edge represents the gene mapped to the pathway in (A,C).
Figure 6

Bar plots for representative terms of GO enrichment analysis. The GO terms for up-regulated DEGs (A) and down-regulated DEGs (B) were reduced using the REVIGO tool and shown in bar plots. The different aspects of representative GO terms are shown in different colors. BP: biological process; MF: molecular function; CC: cellular component.
Figure 7

The diagram of the up- and down-regulated genes in the signaling pathways of fatty acid metabolism and glucose metabolism in the livers of the overfed group. The letters in italic and red represent up-regulated genes, the letters in italic and green represent down-regulated genes.
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Int. J. Mol. Sci.
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