FIGURE SUMMARY
Title

Knockdown of Matrix Metallopeptidase 9 Inhibits Metastasis of Oral Squamous Cell Carcinoma Cells in a Zebrafish Xenograft Model

Authors
Wen, J., Yin, P., Li, L., Kang, G., Ning, G., Cao, Y., Gao, F., Su, Y., Wu, Y., Zhang, X.
Source
Full text @ Biomed Res. Int.
Figure 1

MMP-9 gene knockdown in OSCC cells. (a) The red fluorescence indicated the transfected cells (magnification, ×400). (b) Compared with the control, MMP-9 expression at the protein level was reduced in MMP-9/shRNA groups by immunocytochemistry analysis. Bar = 50 μm. (c) The effect of decreasing MMP-9 expression at the RNA level was detected by RT-PCR. ∗P < 0.05 and ∗∗P < 0.01 as compared with the control. Bar = 200 mm.
Figure 2

Knockdown of MMP-9 could slow down the growth of OSCC cells (CAL-27 and SCC-15 cells) in a time-dependent manner by MTT assay. ∗P < 0.05 and ∗∗P < 0.01 as compared with the control.
Figure 3

Knockdown of MMP-9 could inhibit the colony formation of OSCC cells (CAL-27 and SCC-15 cells) compared with the control. ∗P < 0.05 as compared with the control.
Figure 4

Knockdown of MMP-9 could decrease the invasive ability of OSCC cells (CAL-27 and SCC-15 cells) by transwell invasive assay (magnification, ×400). ∗∗∗P < 0.001 as compared with the control. Bar = 50 mm.
Figure 5

Knockdown of MMP-9 decreased the metastatic distance and number of CAL-27 cells in the zebrafish xenograft model. A red fluorescent cluster indicated a tumor mass, and the foci marked by white arrows indicated the metastatic tumor cells or lesions that have escaped from the yolk sac. (a, b) The bright-field and fluorescent pictures of whole-body, head, distal tail regions of zebrafish injected with CAL-27/LVRU6MP and CAL-27/MMP-9/shRNA cells at 3 dpi (n = 120; magnification, ×32, ×63). (c, e) Knockdown of MMP-9 reduced the invasive average distance, maximal distance, and number of disseminated cells or lesions at 3 dpi. ∗P < 0.05 and ∗∗P < 0.01 as compared with the control. Bar = 0.5 mm.
Figure 6

Knockdown of MMP-9 reduced the metastatic distance and number of SCC-15 cells and decreased the metastasis rate in the zebrafish xenograft model. (a, b) The bright-field and fluorescent pictures of whole-body, head, distal tail regions of zebrafish injected with SCC-15/LVRU6MP (n = 230; magnification, ×32, ×63) and SCC-15/MMP-9/shRNA cells at 5 dpi (n = 290; magnification, ×32, ×63). (c, e) Knockdown of MMP-9 decreased the invasive average distance and number of disseminated cells or lesions at 5 dpi and inhibited the metastasis rate from 3 dpi to 6 dpi. ∗P < 0.05 and ∗∗P < 0.01 as compared with the control. Bar = 0.5 mm.
Acknowledgments
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