PUBLICATION
Knockdown of Matrix Metallopeptidase 9 Inhibits Metastasis of Oral Squamous Cell Carcinoma Cells in a Zebrafish Xenograft Model
- Authors
- Wen, J., Yin, P., Li, L., Kang, G., Ning, G., Cao, Y., Gao, F., Su, Y., Wu, Y., Zhang, X.
- ID
- ZDB-PUB-200510-7
- Date
- 2020
- Source
- BioMed Research International 2020: 4350783 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Animals
- Cell Line, Tumor
- Gene Knockdown Techniques*
- Head and Neck Neoplasms*/enzymology
- Head and Neck Neoplasms*/genetics
- Head and Neck Neoplasms*/pathology
- Heterografts
- Humans
- Matrix Metalloproteinase 9*/genetics
- Matrix Metalloproteinase 9*/metabolism
- Neoplasm Metastasis
- Neoplasm Proteins*/genetics
- Neoplasm Proteins*/metabolism
- Neoplasm Transplantation
- Squamous Cell Carcinoma of Head and Neck*/enzymology
- Squamous Cell Carcinoma of Head and Neck*/genetics
- Squamous Cell Carcinoma of Head and Neck*/pathology
- Zebrafish
- PubMed
- 32382550 Full text @ Biomed Res. Int.
Citation
Wen, J., Yin, P., Li, L., Kang, G., Ning, G., Cao, Y., Gao, F., Su, Y., Wu, Y., Zhang, X. (2020) Knockdown of Matrix Metallopeptidase 9 Inhibits Metastasis of Oral Squamous Cell Carcinoma Cells in a Zebrafish Xenograft Model. BioMed Research International. 2020:4350783.
Abstract
Destruction of extracellular matrix (ECM) is one of the basic steps of tumor invasion and metastasis. Matrix metalloproteinase (MMP) 9, a kind of zinc-ion-dependent endopeptidase, can degrade almost all protein components in the ECM, destroy the histological barrier of tumor cell invasion, and play a key role in tumor invasion and metastasis. The role of MMP-9 in tumor invasion and metastasis has attracted increasing attention and is considered the main proteolytic enzyme in this process. Although the overexpression of MMP-9 was detected in Oral squamous cell carcinoma (OSCC) tissues, further basic studies in vivo and in vitro are needed to investigate the role of MMP-9 in OSCCs and provide scientific validation. In this research, we developed a novel OSCC zebrafish xenograft model to study the role of the MMP-9 gene in oral carcinogenesis. Firstly, the MMP-9/shRNA lentiviral clone and control virus were constructed and transfected into OSCC cells. Then, the decreasing expression of MMP-9 was verified by RT-PCR and immunocytochemistry. Cell proliferation was detected by MTT assay. Colony formation was evaluated by colony formation assay. Cell invasion was evaluated using transwell invasion assay in vitro. In addition, OSCC cells with MMP-9/shRNA knockdown and control vector were injected into zebrafish and an OSCC tumor model in zebrafish was established to evaluate invasion and metastasis in vivo. Knockdown of MMP-9 gene by shRNA could inhibit OSCC cell growth and clone formation and markedly suppress cell invasion in vitro. And the knockdown of the MMP-9 gene could also significantly decrease the metastatic distance and number of metastatic tumor cells or lesions in vivo and suppress the metastasis rate in xenografted zebrafish. Taken together, these evidences indicated that the knockdown of MMP-9 might suppress OSCC cell invasion and metastasis in vivo and in vitro. The MMP-9 gene may be a promising therapeutic target for OSCCs in the future.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping