CRISPR

CRISPR4-cdh5

ID

ZDB-CRISPR-200107-1

Name

CRISPR4-cdh5

Previous Names

None

Target

cdh5 (1)

Sequence

5' - GGGACCTGCACTCTATGCCA - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

This CRISPR was designed to be specific for the AB line. (The TU allele has as SNP (C->T transition) at position 13 of the recognition sequence.)

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
ubs25	cdh5

Expression

Gene expression in Wild Types + CRISPR4-cdh5

No data available

Phenotype

Phenotype resulting from CRISPR4-cdh5

No data available

Phenotype of all Fish created by or utilizing CRISPR4-cdh5

Phenotype	Fish	Conditions	Figures
blood vessel endothelial cell cell-cell junction ab1-tjp1 labeling decreased distribution, abnormal	cdh5^ubs25/ubs25	standard conditions	Fig. 7 from Paatero et al., 2018
pericardium edematous, abnormal	cdh5^ubs25/ubs25	standard conditions	Fig. S5 from Paatero et al., 2018
blood circulation decreased occurrence, abnormal	cdh5^ubs25/ubs25	standard conditions	Fig. S5 from Paatero et al., 2018
blood vessel endothelial cell cell-cell junction ab1-tjp1 labeling decreased amount, abnormal	cdh5^ubs25/ubs25	standard conditions	Fig. 7 from Paatero et al., 2018
posterior cardinal vein increased size, abnormal	cdh5^ubs25/ubs25; s843Tg	standard conditions	Fig. S5 from Paatero et al., 2018
intersegmental vessel blood vessel morphogenesis process quality, abnormal	cdh5^ubs25/ubs25; ubs1Tg	standard conditions	Fig. 7 from Paatero et al., 2018
intersegmental vessel cell-cell junction organization process quality, abnormal	cdh5^ubs25/ubs25; ubs1Tg	standard conditions	Fig. 7 from Paatero et al., 2018
endothelial tip cell separated from blood vessel endothelial cell, abnormal	cdh5^ubs25/ubs25; ubs18Tg; ubs3Tg	standard conditions	Fig. S5 from Paatero et al., 2018
dorsal longitudinal anastomotic vessel cell-cell junction organization process quality, abnormal	cdh5^ubs25/ubs25; ubs18Tg; ubs3Tg	standard conditions	Fig. 7 from Paatero et al., 2018
blood vessel endothelial cell cell-cell junction organization process quality, abnormal	cdh5^ubs25/ubs25; ubs18Tg; ubs3Tg	standard conditions	Fig. 7 from Paatero et al., 2018
intersegmental vessel disconnected, abnormal	cdh5^ubs25/ubs25; ubs18Tg; ubs3Tg	standard conditions	Fig. S5 from Paatero et al., 2018

Citations