PUBLICATION
Junction-based lamellipodia drive endothelial cell rearrangements in vivo via a VE-cadherin-F-actin based oscillatory cell-cell interaction
- Authors
- Paatero, I., Sauteur, L., Lee, M., Lagendijk, A.K., Heutschi, D., Wiesner, C., Guzmán, C., Bieli, D., Hogan, B.M., Affolter, M., Belting, H.G.
- ID
- ZDB-PUB-190117-8
- Date
- 2018
- Source
- Nature communications 9: 3545 (Journal)
- Registered Authors
- Affolter, Markus, Belting, Heinz-Georg Paul (Henry), Hogan, Ben M., Paatero, Ilkka, Sauteur, Loïc
- Keywords
- none
- MeSH Terms
-
- Actins/metabolism*
- Animals
- Animals, Genetically Modified
- Antigens, CD/physiology*
- Cadherins/physiology*
- Cell Communication/physiology
- Cell Movement/physiology*
- Embryo, Nonmammalian
- Endothelial Cells/physiology*
- Intercellular Junctions/physiology
- Pseudopodia/physiology*
- Zebrafish Proteins/metabolism
- Zonula Occludens-1 Protein/metabolism
- PubMed
- 30171187 Full text @ Nat. Commun.
Citation
Paatero, I., Sauteur, L., Lee, M., Lagendijk, A.K., Heutschi, D., Wiesner, C., Guzmán, C., Bieli, D., Hogan, B.M., Affolter, M., Belting, H.G. (2018) Junction-based lamellipodia drive endothelial cell rearrangements in vivo via a VE-cadherin-F-actin based oscillatory cell-cell interaction. Nature communications. 9:3545.
Abstract
Angiogenesis and vascular remodeling are driven by extensive endothelial cell movements. Here, we present in vivo evidence that endothelial cell movements are associated with oscillating lamellipodia-like structures, which emerge from cell junctions in the direction of cell movements. High-resolution time-lapse imaging of these junction-based lamellipodia (JBL) shows dynamic and distinct deployment of junctional proteins, such as F-actin, VE-cadherin and ZO1, during JBL oscillations. Upon initiation, F-actin and VE-cadherin are broadly distributed within JBL, whereas ZO1 remains at cell junctions. Subsequently, a new junction is formed at the front of the JBL, which then merges with the proximal junction. Rac1 inhibition interferes with JBL oscillations and disrupts cell elongation-similar to a truncation in ve-cadherin preventing VE-cad/F-actin interaction. Taken together, our observations suggest an oscillating ratchet-like mechanism, which is used by endothelial cells to move over each other and thus provides the physical means for cell rearrangements.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
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