- Title
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Expression and function of lncRNA MALAT-1 in the embryonic development of zebrafish
- Authors
- Wu, M., Zhang, S., Chen, X., Xu, H., Li, X.
- Source
- Full text @ Gene
Expression of MALAT-1 in zebrafish embryos at different stages of development. A–H: In situ hybridization showing MALAT-1 expression in zebrafish embryos at different stages of development. I: The relative expression of MALAT-1 in zebrafish embryos at different stages of development determined by using qPCR. MALAT-1 levels were normalized to the expression of Rpl13ɑ. The data are shown as the means ± SD from three independent experiments with triplicate. Asterisk denote a response that is significantly different from the control (*p < 0.05, **p < 0.01). EXPRESSION / LABELING:
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ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions. |
Morpholino knockdown of MALAT-1 and validation. A: Frequency of morphant phenotypes in injected embryos. B: qPCR monitoring MALAT-1 in control-MO embryos (48 hpf) that had been injected with the indicated MOs. MALAT-1 levels were normalized to the expression of Rpl13ɑ. The data are shown as the means ± SD from three independent experiments with triplicate. Asterisk denote a response that is significantly different from the control (*p < 0.05, **p < 0.01). C: In situ hybridization showing MALAT-1 expression in MALAT-1-MO knockdown and control-MO embryos (48 hpf). D: Gene architecture of MALAT-1, showing the probe of in situ hybridization (yellow box), qPCR probe (purple box), test-primer (green box), cutting site, and transcription direction (arrow direction). (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.) |
Comparison of MALAT-1-MO and control-MO phenotypes at different developmental stages of zebrafish embryos. A: Embryos at 24 hpf that had been injected with MALAT-1-MO and control-MO, respectively. B: Embryos at 96 hpf that had been injected with MALAT-1-MO and control-MO, respectively. C: Embryos at 72 hpf that had been injected with MALAT-1-MO and control-MO, respectively. The morphant revealed defects in ear. Yellow arrow indicates zebrafish ear and red arrow points to otic capsule and otolith. Abbreviations: oc: otic capsule; ot: otolith. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.) |
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ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions. |
Reprinted from Gene, 680, Wu, M., Zhang, S., Chen, X., Xu, H., Li, X., Expression and function of lncRNA MALAT-1 in the embryonic development of zebrafish, 65-71, Copyright (2018) with permission from Elsevier. Full text @ Gene