PUBLICATION

Expression and function of lncRNA MALAT-1 in the embryonic development of zebrafish

Authors
Wu, M., Zhang, S., Chen, X., Xu, H., Li, X.
ID
ZDB-PUB-180925-10
Date
2018
Source
Gene   680: 65-71 (Journal)
Registered Authors
Xu, Hui, Zhang, Shuqiang
Keywords
Embryonic development, LncRNA MALAT-1, Zebrafish
MeSH Terms
  • Animals
  • Brain Chemistry
  • Central Nervous System/chemistry
  • Central Nervous System/growth & development
  • Epigenesis, Genetic
  • Eye/chemistry
  • Eye/growth & development
  • Gene Expression Profiling/methods*
  • Gene Expression Regulation, Developmental
  • Gene Knockdown Techniques
  • Gene Regulatory Networks
  • Heart/growth & development
  • Muscle Development
  • Muscles/chemistry
  • Myocardium/chemistry
  • RNA, Long Noncoding/genetics*
  • Tissue Distribution
  • Zebrafish/genetics
  • Zebrafish/growth & development*
PubMed
30248354 Full text @ Gene
Abstract
Long non-coding RNAs (lncRNAs) are RNA molecules with a transcript length of >200 nt which do not encode proteins, however, they have important functions in the form of RNA at transcriptional level, post-transcriptional level, and epigenetics. MALAT-1 is the first discovered lncRNA in non-small cell lung cancer and its orthologs have been found in zebrafish in the recent years. The present study aimed to determine the expression and possible function of MALAT-1 in the embryonic development of zebrafish. The results of qPCR or in situ hybridization showed that MALAT-1 was dynamically expressed in zebrafish embryonic development and it was widely expressed in brain, eye, heart, and muscle of adult fish. By morpholino knockdown of MALAT-1, Zebrafish embryos were found to have smaller body curvature, smaller eyes, enlarged pericardium, and reduced pigmentation. In particular, after morpholino knockdown of MALAT-1, the morphologically defective otic capsule was found to be smaller than the control fish. These results suggest that MALAT-1 may play a key role in the embryonic nervous system development of zebrafish. In addition, we also determined the expressions of seven development-related genes (Scyl1, egr1, oc90, vsx1, nkx2.5, vmhc, and gata4) after MALAT-1 knockdown and found that four genes egr1, nkx2.5, gata4, and vmhc were up-regulated, suggesting a potential regulatory role of MALAT-1 on these development-related genes.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping