- Title
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Fatty Acid Binding Protein 11a Is Required for Brain Vessel Integrity in Zebrafish
- Authors
- Zhang, J., Qi, J., Wu, S., Peng, L., Shi, Y., Yang, J., Yin, Z., Gao, Y., Wang, C., Gong, J., Zhang, H., Zhang, J., Liu, D.
- Source
- Full text @ Front. Physiol.
Whole mount in situ hybridization analysis of fabp11a in zebrafish embryos. (A) 20 hpf, lateral view. (B) 24 hpf, lateral view. Red asterisk indicates mid-cerebral vein (MCeV). (B') 24 hpf, lateral view. Red asterisk indicates mid-cerebral vein (MCeV). (C) 30 hpf, lateral view. Red arrowhead indicates MCeV; Red asterisk indicates primordial hindbrain channel (PHBC). (C') 30 hpf, lateral view. Red arrowhead indicates MCeV; Red asterisk indicates PHBC. (C'') 30 hpf, dorsal view. Red arrowhead indicates MCeV; Red asterisks indicate PHBC. (D) 36 hpf, lateral view. Red arrowhead indicates MCeV; Red asterisk indicates primordial hindbrain channel (PHBC). (D') 36 hpf, lateral view. Red arrowhead indicates MCeV; Red asterisk indicates PHBC. (D'') 36 hpf, dorsal view. Red arrowhead indicates MCeV; Red asterisks indicate PHBC. (E,E') 48 hpf, lateral view. Red arrowhead indicates brain vessels. (E”) 48 hpf, dorsal view. Red arrowhead indicates brain vessels. (F,F') 72 hpf, lateral view. Red arrowhead indicates brain vessels. (F'') 72 hpf, dorsal view. Red arrowhead indicates brain vessels. EXPRESSION / LABELING:
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fabp11a knockout leads to zebrafish brain hemorrhage. (A) Confocal imaging analysis of brain vascular morphology in control and fabp11a mutants Tg(kdrl:EGFP) embryos at 48 hpf. (B) Microscopy analysis of control embryos and fabp11a mutants in bright field at 48 hpf. Blue arrowheads indicate hemorrhage in zebrafish head. (C) Microscope analysis of control embryos and fabp11a mutants with O-dianisidine staining in bright field at 48 hpf. Blue arrowheads indicate hemorrhage in zebrafish head. (D) Fluorescent microscopy imaging of Tg(gata1:DsRed) embryos at 48 hpf. Scale bar: 20 μm. |
Zebrafish fabp11a mutants present disrupted brain vessel integrity. (A–B'') Confocal imaging analysis of fabp11a mutants and control Tg(kdrl:EGFP) embryos intracardiac injected with Rhodamine-conjugated dextran at 72 hpf. (C) Quantification of the permeability of the selected vessels in fabp11a mutants and control Tg(kdrl:EGFP) embryos using E/I = extravascular fluorescence intensity /Intravascular fluorescence intensity. T-test, ***P < 0.001, scale bar: 20 μm. EXPRESSION / LABELING:
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ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions. PHENOTYPE:
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fabp11a knockdown leads to zebrafish brain hemorrhage. (A, B) Microscopy analysis of control embryos and fabp11a morphants in bright field at 72 hpf. Blue arrowheads indicate hemorrhage in zebrafish head. (C-D'') Confocal imaging analysis of fabp11a morphants and control Tg(kdrl:EGFP) embryos intracardiac injected with Rhodamine-conjugated dextran at 72 hpf. (E) Fabp11a mRNA injection rescued the blood vessel integrity defects caused by fabp11a deficiency, χ2 test, ***P<0.001. Scale bar: 20 µm. EXPRESSION / LABELING:
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Pharmacological inhibition of mTOR, eNOS and P38 signaling impairs branching angiogenesis of brain vessels. (A-D) Confocal imaging analysis of control, rapamycin treated, L-NAME treated and SB203580 treated Tg(kdrl:EGFP) embryos. (B) Reverse transcription polymerase chain reaction analysis of fabp11a expression in control, rapamycin treated, L-NAME treated and SB203580 treated Tg(kdrl:EGFP) embryos. Scale bar: 20 µm. EXPRESSION / LABELING:
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