Fig. 3

Transgenic wt1b:GFP Embryos with Green Fluorescence in the Developing Kidney. Overlays of dorsal (A) or lateral (B) transmission and fluorescence images are shown with anterior to the left. np, nephron primordium; pt, pronephric tubule; so, somite.

EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage: Prim-5
Fig. 4

Knockdown of wt1a Disrupts Embryonic Kidney Development. Representative, extended depth of focus images from time-lapse recordings. In control morpholino injected embryos, kidney development shows normal progress with growing tubules and nephron primordia which start to fuse at the midline. In contrast, wt1a morphants fail to form proper nephron primordia and a massive amount of GFP positive cells are outside of the pronephric field. (np, nephron primordium; pt, pronephric tubule).

EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: 20-25 somites to Prim-5
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: 20-25 somites to Prim-5
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Vis. Exp.
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