- Title
-
Acute Exposure to Tris(1,3-dichloro-2-propyl) Phosphate (TDCIPP) Causes Hepatic Inflammation and Leads to Hepatotoxicity in Zebrafish
- Authors
- Liu, C., Su, G., Giesy, J.P., Letcher, R.J., Li, G., Agrawal, I., Li, J., Yu, L., Wang, J., Gong, Z.
- Source
- Full text @ Sci. Rep.
Changes of liver histology of male zebrafish after exposure to 1 mg/L TDCIPP for 4 days. Liver sections were stained by hematoxylin and eosin. (A) representative liver section from the DMSO vehicle control group. (B,C) Two representative liver sections from two individual fish of the same TDCIPP group with a moderate effect (B) and a severe effect (C). The left images have a magnification of 200X and the right images have a magnification of 400X. Several features are exampled by arrows of different colors: neutrophils (black); apoptosis (red); hepatic vacuolization (green). |
Dose- (A) and time-dependent (B) increase of neutrophils in the livers of Tg(lysC:dDsRed) zebrafish larvae in response to TDCIPP exposure.Images were captured with a digital camera attached to a Carl Zeiss LSM 510 Meta fluorescent microscope. (C) Representative images from control group and 4-day TDCIPP (1 mg/L) exposure group. The livers are outlined with white lines. Values represent mean ± SEM. Significant difference between the two groups were observed: P < 0.05. |
Dose- (A) and time-dependent (B) increase of liver size in Tg(fabp10a:DsRed) zebrafish larvae after exposure to TDCIPP.Images were captured with a digital camera attached to a Carl Zeiss LSM 510 Meta fluorescent microscope. Liver sizes were measured based on the 2D image using the ImageJ software. (C) Representative images from control group and 4-day TDCIPP (1 mg/L) exposure group Values represent mean ± SEM. Significant difference between the two groups were observed: P < 0.05. |