Expression of kcnq in zebrafish brain (A), ear (B) and heart (C). Lanes labeled M are the 100 bp ladder molecular standards. Lane 9 and 10, respectively, is a positive control with β-actin, and a negative control without RNA.

qRTPCR of Kcnq channel transcripts in brain, heart, and ear. Real time PCR thresholds (Delta CT) were first normalized to GAPDH or β-actin for each tissue. These data were then plotted relative to the lowest expressed mRNA in each tissue using a logarithmic scale. Depicted also is +/-1.0 standard error.

Expression of kcnq4 transcripts from chromosome 19. A. PCR products from reverse transcriptase PCR reactions using primers (Table 1) for kcnq 4. Lanes 2 and 5 are brain mRNA products, while lanes 3 and 4 are zebrafish ear mRNA-derived products. Lane 1 is a 100 bp ladder standard. B. PCR products from reverse-transcriptase PCR reactions using primers (Table 1) for Chr 19 (Lanes 2 and 3) and the Zv9_NA546 scaffold kcnq 4 (lanes 4 and 5) of mRNA from zebrafish heart. Lane 1 is a 500 bp ladder standard.

A. Alignment of the partial zebrafish kcqn4 sequence (A), with KCNQ4 sequences from chimpanzee (B), frog (C) and human (D). The sequences on top of the KCNQ4 groups are the translation of the sequences that we obtained from the sequencing of PCR products (Table 2). The carboxyl region that contains the sequences implicated in the assembly of homo- vs. hetero-tetramers is indicated with bars, with the Head-Linker-Tail designations. B. Alignment of the translation of the Chr 19 sequence of zebrafish Kcnq4 (A) with the amino termini of Frog (B), Chimpanzee (C) and Human (D).

Kcnq4 protein expression in zebrafish brain and ear tissues. Western blots of Brain (Lane A) and Ear (Lane B) extracts probed with rabbit antibody to zebrafish Kcnq4. Lane C is zebrafish ear tissue probed with antisera containing 100 ng/mL of the synthetic peptide used for immunogen production added.