FIGURE SUMMARY
Title

ZebIAT, an image analysis tool for registering zebrafish embryos and quantifying cancer metastasis

Authors
Annila, T., Lihavainen, E., Marques, I.J., Williams, D.R., Yli-Harja, O., and Ribeiro, A.
Source
Full text @ BMC Bioinformatics
Fig. 1

Example of a reference embryo, with organs marked. For easier visualization, only the borders of the masks of the organs are shown.
Fig. 2

Segmentation procedure. Top left: Image from the green fluorescence channel. Top right: Image filtered with LoG. Middle left: Result from LoG edge detection. Middle right: Result from Otsu′s threshold. Bottom left: Segmented image after all steps, including morphological closing. Bottom right: Outline of the fish. For illustration purposes, the images are presented with inverted grayscale values.
Fig. 3

Segmentation of a DIC image. Top left: grayscale image with inverted grayscale values. Top right: Image filtered with LoG. Middle left: Result from LoG edge detection. Middle right: Result from Otsu′s threshold. Bottom left: Segmented image after all steps, including morphological closing. Bottom right: Outline of the fish.
Fig. 5

Registration procedure. Top: Reference fish with 1 day after injection of cancer cells. Middle: Fish to be registered. Bottom: Registered fish. For illustration purposes, the images are presented with inverted grayscale values.
Fig. 6

Spot detection. Top: red channel of the image. Bottom: segmented spots. Gamma correction was used in the two images to improve the visibility of the spots. For illustration purposes, the images are presented with inverted grayscale values.
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Bioinformatics
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