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Figure Caption
Fig. 2
Segmentation procedure. Top left: Image from the green fluorescence channel. Top right: Image filtered with LoG. Middle left: Result from LoG edge detection. Middle right: Result from Otsu′s threshold. Bottom left: Segmented image after all steps, including morphological closing. Bottom right: Outline of the fish. For illustration purposes, the images are presented with inverted grayscale values.
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