Forrester et al., 2012 - Myelopoiesis and Myeloid Leukaemogenesis in the Zebrafish. Advances in hematology   2012:358518 Full text @ Adv. Hematol.

Figure 1

Overview of zebrafish developmental myelopoiesis, key transgenic lines, and lineage identification tools labelling myeloid cell populations during developmental haematopoiesis. (Transgenic lines are shown in green, other specific lineage identifiers are in blue.) PM: primitive myelopoiesis; EMP: erythromyeloid progenitors; HSPCs: haematopoietic stem and progenitor cells; CMP: common myeloid progenitor; CLP: common lymphoid progenitor; MEP: megakaryocyte/erythroid progenitor; GMP: granulocyte/monocyte progenitor; PHA: peanut haemaglutinin. **Denotes lineages only demonstrated in adult zebrafish. Lineage intermediates are shown for clarity but are yet to be isolated as distinct populations in zebrafish.

Figure 2

Schematic of in vivo cell proliferation assay in xenotransplanted zebrafish embryos. Human leukemia cells are fluorescently labelled with a cell tracking dye. Approximately 25–50 fluorescently labelled cells are microinjected into the yolk sac of 48 hpf casper embryos. Embryos are screened using fluorescent microscopy for the presence of a fluorescent mass at the site of injection. Positive embryos are divided into two groups; one of which is maintained at 35C for 24 h, and the other group is maintained for until the time point of interest with or without drug exposure. At the end of each time point embryos are enzymatically dissociated to a single cell suspension and the number of fluorescent cells in the suspension is counted using a semiautomated macro in Image J (NIH, Bethesda, MD). The number of fluorescent cells present at the later time point divided by the number of fluorescent cells present at 24 h represents the fold increase in cell number. Adapted from Corkery et al. [90].

ZFIN wishes to thank the journal Advances in hematology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Adv. Hematol.