FIGURE

Figure 2

ID

ZDB-FIG-190723-1988

Publication

Forrester et al., 2012 - Myelopoiesis and Myeloid Leukaemogenesis in the Zebrafish

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Figure 2

Schematic of in vivo cell proliferation assay in xenotransplanted zebrafish embryos. Human leukemia cells are fluorescently labelled with a cell tracking dye. Approximately 25–50 fluorescently labelled cells are microinjected into the yolk sac of 48 hpf casper embryos. Embryos are screened using fluorescent microscopy for the presence of a fluorescent mass at the site of injection. Positive embryos are divided into two groups; one of which is maintained at 35C for 24 h, and the other group is maintained for until the time point of interest with or without drug exposure. At the end of each time point embryos are enzymatically dissociated to a single cell suspension and the number of fluorescent cells in the suspension is counted using a semiautomated macro in Image J (NIH, Bethesda, MD). The number of fluorescent cells present at the later time point divided by the number of fluorescent cells present at 24 h represents the fold increase in cell number. Adapted from Corkery et al. [90].

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Adv. Hematol.