de Jong et al., 2012 - Histocompatibility and Hematopoietic Transplantation in the Zebrafish. Advances in hematology   2012:282318 Full text @ Adv. Hematol.

Figure 1

Flow cytometry analysis of zebrafish whole kidney marrow from a marrow transplant recipient. Zebrafish transplant recipients were irradiated and injected with 5 × 105 marrow cells from a transgenic β-actin:GFP donor. Whole kidney marrow from a representative recipient was dissected 3 months later and analyzed by flow cytometry. (a) The forward scatter (FSC) versus side scatter (SSC) profile of zebrafish whole kidney marrow shows four cell populations: erythroid, lymphoid, myeloid, and precursor cells. (b) Histograms for GFP expression of cells within the lymphoid, myeloid and precursor gates show multilineage engraftment with GFP+ donor cells (blue lines). The red lines show GFP expression in a wild-type-negative control fish.

Figure 2

Direct visualization of engrafted GFP+ and mCherry+ marrow donor cells in casper recipients. 40 × 103 WKM cells from a transgenic ubiquitin:GFP donor were mixed with 80 × 103 WKM cells from a transgenic ubiquitin:mCherry donor and injected into the circulation of a casper recipient fish. The photos are taken 4 weeks after transplantation and show engraftment of (a) GFP+ and (b) mCherry+ cells in the kidney (white arrows).

Figure 3

Survival of different zebrafish lines in response to radiation. Kaplan-Meier survival curves are shown for four different zebrafish strains after irradiation with a total dose of 25 Gy, delivered in two equal fractions of 12.5 Gy separated by 24 hours. Twenty one fish were irradiated in each group. CG1 is a clonal homozygous diploid fish line generated by parthenogenesis [21, 38]. UDA, UXA2, and UBA are inbred zebrafish lines derived from a single mating pair of AB parents [35]. Each line was named for the homozygous class I MHC gene at its chromosome 19 locus. The results demonstrate 100% mortality for the CG1 fish by day 22, and by day 37 for the UDA fish. In contrast, the UBA and UXA2 fish lines both had approximately 80% survival at 40 days after irradiation.

ZFIN wishes to thank the journal Advances in hematology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Adv. Hematol.