Transgenic Construct
Tg(E1B:Hsa.ATXN1[30Q],E1B:GAP-mScarlet)
- ID
- ZDB-TGCONSTRCT-220822-2
- Name
- Tg(E1B:Hsa.ATXN1[30Q],E1B:GAP-mScarlet)
- Previous Names
- None
- Type
- engineered_plasmid
- Regulatory Regions
- Coding Sequences
- Contains Sequences
- None
- Note
-
This construct was designed to express a non-pathological human patient-derived allele of Ataxin 1 in zebrafish Purkinje cells. The mutant sequence contains a non-pathological polyglutamine stretch of 30 glutamine residues ( Atx1[30Q] ), interrupted by two histidine residues. An HA-tag is fused to the N-terminus for immunohistochemical detection of the transgene product
Plasmid Map
None
Genomic Features
That Utilize tg(e1b:hsa.atxn1[30q],e1b:gap-mscarlet) Construct
| Genomic Feature | Affected Genomic Regions |
|---|---|
| bz21Tg |
Transgenics
That Utilize tg(e1b:hsa.atxn1[30q],e1b:gap-mscarlet) Construct
| Fish | Affected Genomic Regions | Phenotype | Gene Expression |
|---|---|---|---|
| bz12Tg; bz21Tg/+ |
|
Figure 3
from
Elsaey et al., 2021
|
3 figures
from
Elsaey et al., 2021
|
| bz21Tg/+ |
|
Figure 5
from
Elsaey et al., 2021
|
Figure 4
from
Elsaey et al., 2021
|
Sequence Information
Other Construct Pages
None
Citations