ZFIN ID: ZDB-PUB-980415-5
SCL/Tal-1 transcription factor acts downstream of cloche to specify hematopoietic and vascular progenitors in zebrafish
Liao, E.C., Paw, B.H., Oates, A.C., Pratt, S.J., Postlethwait, J.H., and Zon, L.I.
Date: 1998
Source: Genes & Development   12: 621-626 (Journal)
Registered Authors: Liao, Eric, Oates, Andrew, Paw, Barry, Postlethwait, John H., Pratt, Stephen J., Zon, Leonard I.
Keywords: clo; zebrafish; hematopoiesis; vasculogenesis
MeSH Terms:
  • Amino Acid Sequence
  • Animals
  • Basic Helix-Loop-Helix Transcription Factors
  • Chromosome Mapping
  • Conserved Sequence
  • DNA-Binding Proteins/genetics*
  • DNA-Binding Proteins/metabolism
  • Embryo, Nonmammalian
  • Endothelium, Vascular/embryology*
  • Gene Expression Regulation, Developmental
  • Hematopoietic Stem Cells/physiology*
  • Molecular Sequence Data
  • Mutation
  • Proto-Oncogene Proteins*
  • Transcription Factors*
  • Zebrafish/embryology*
  • Zebrafish/genetics*
  • Zebrafish Proteins*
PubMed: 9499398 Full text @ Genes & Dev.
SCL/Tal-1 is a transcription factor necessary for hematopoietic stem cell differentiation. Although SCL is also expressed in endothelial and neural progenitors, SCL function in these cells remains unknown. In the zebrafish mutant cloche (clo), SCL expression is nearly abolished in hematopoietic and vascular tissues. Correspondingly, it was shown previously that clo fails to differentiate blood and angioblasts. Genetic analysis demonstrates that the clo mutation is not linked to the SCL locus. Forced expression of SCL in clo embryos rescues the blood and vascular defects, suggesting that SCL acts downstream of clo to specify hematopoietic and vascular differentiation.