PUBLICATION
An improved Erk biosensor detects oscillatory Erk dynamics driven by mitotic erasure during early development
- Authors
- Wilcockson, S.G., Guglielmi, L., Araguas Rodriguez, P., Amoyel, M., Hill, C.S.
- ID
- ZDB-PUB-230916-56
- Date
- 2023
- Source
- Developmental Cell 58(23): 2802-2818.e5 (Journal)
- Registered Authors
- Hill, Caroline
- Keywords
- Drosophila, Erk, Fgf signaling, kinase translocation reporter, mitosis, zebrafish
- MeSH Terms
-
- Animals
- Biosensing Techniques*
- Drosophila/metabolism
- Extracellular Signal-Regulated MAP Kinases*/metabolism
- Fibroblast Growth Factors/metabolism
- Signal Transduction
- Zebrafish/metabolism
- PubMed
- 37714159 Full text @ Dev. Cell
Citation
Wilcockson, S.G., Guglielmi, L., Araguas Rodriguez, P., Amoyel, M., Hill, C.S. (2023) An improved Erk biosensor detects oscillatory Erk dynamics driven by mitotic erasure during early development. Developmental Cell. 58(23):2802-2818.e5.
Abstract
Extracellular signal-regulated kinase (Erk) signaling dynamics elicit distinct cellular responses in a variety of contexts. The early zebrafish embryo is an ideal model to explore the role of Erk signaling dynamics in vivo, as a gradient of activated diphosphorylated Erk (P-Erk) is induced by fibroblast growth factor (Fgf) signaling at the blastula margin. Here, we describe an improved Erk-specific biosensor, which we term modified Erk kinase translocation reporter (modErk-KTR). We demonstrate the utility of this biosensor in vitro and in developing zebrafish and Drosophila embryos. Moreover, we show that Fgf/Erk signaling is dynamic and coupled to tissue growth during both early zebrafish and Drosophila development. Erk activity is rapidly extinguished just prior to mitosis, which we refer to as mitotic erasure, inducing periods of inactivity, thus providing a source of heterogeneity in an asynchronously dividing tissue. Our modified reporter and transgenic lines represent an important resource for interrogating the role of Erk signaling dynamics in vivo.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping