PUBLICATION
The zebrafish mutant dreammist implicates sodium homeostasis in sleep regulation
- Authors
- Barlow, I.L., Mackay, E., Wheater, E., Goel, A., Lim, S., Zimmerman, S., Woods, I., Prober, D.A., Rihel, J.
- ID
- ZDB-PUB-230810-41
- Date
- 2023
- Source
- eLIFE 12: (Journal)
- Registered Authors
- Barlow, Ida, Prober, David, Rihel, Jason, Woods, Ian G., Zimmerman, Steve
- Keywords
- genetic screen, neuroscience, sleep, sleep homeostasis, sodium-potassium pump, zebrafish
- MeSH Terms
-
- Animals
- Homeostasis
- Humans
- Phosphoproteins/metabolism
- Sleep/genetics
- Sodium*/metabolism
- Sodium-Potassium-Exchanging ATPase/genetics
- Sodium-Potassium-Exchanging ATPase/metabolism
- Zebrafish*/genetics
- Zebrafish*/metabolism
- PubMed
- 37548652 Full text @ Elife
Citation
Barlow, I.L., Mackay, E., Wheater, E., Goel, A., Lim, S., Zimmerman, S., Woods, I., Prober, D.A., Rihel, J. (2023) The zebrafish mutant dreammist implicates sodium homeostasis in sleep regulation. eLIFE. 12:.
Abstract
Sleep is a nearly universal feature of animal behaviour, yet many of the molecular, genetic, and neuronal substrates that orchestrate sleep/wake transitions lie undiscovered. Employing a viral insertion sleep screen in larval zebrafish, we identified a novel gene, dreammist (dmist), whose loss results in behavioural hyperactivity and reduced sleep at night. The neuronally expressed dmist gene is conserved across vertebrates and encodes a small single-pass transmembrane protein that is structurally similar to the Na+,K+-ATPase regulator, FXYD1/Phospholemman. Disruption of either fxyd1 or atp1a3a, a Na+,K+-ATPase alpha-3 subunit associated with several heritable movement disorders in humans, led to decreased night-time sleep. Since atpa1a3a and dmist mutants have elevated intracellular Na+ levels and non-additive effects on sleep amount at night, we propose that Dmist-dependent enhancement of Na+ pump function modulates neuronal excitability to maintain normal sleep behaviour.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping