PUBLICATION
Caffeine Inhibits Direct and Indirect Angiogenesis in Zebrafish Embryos
- Authors
- Basnet, R.M., Zizioli, D., Muscò, A., Finazzi, D., Sigala, S., Rossini, E., Tobia, C., Guerra, J., Presta, M., Memo, M.
- ID
- ZDB-PUB-210603-7
- Date
- 2021
- Source
- International Journal of Molecular Sciences 22(9): (Journal)
- Registered Authors
- Presta, Marco, Tobia, Chiara
- Keywords
- FGF2, angiogenesis, caffeine, embryonic vascular development, methylxanthines, zebrafish
- MeSH Terms
-
- Animals
- Caffeine/pharmacology*
- Cell Line, Tumor
- Embryo, Nonmammalian
- Embryonic Development/drug effects
- Fibroblast Growth Factor 2/genetics*
- Gene Expression Regulation, Developmental/drug effects
- Heterografts
- Humans
- In Situ Hybridization
- Neovascularization, Pathologic/drug therapy*
- Neovascularization, Pathologic/genetics
- Neovascularization, Pathologic/pathology
- Neovascularization, Physiologic/drug effects*
- Neovascularization, Physiologic/genetics
- Zebrafish/genetics
- Zebrafish/growth & development
- PubMed
- 34063734 Full text @ Int. J. Mol. Sci.
Citation
Basnet, R.M., Zizioli, D., Muscò, A., Finazzi, D., Sigala, S., Rossini, E., Tobia, C., Guerra, J., Presta, M., Memo, M. (2021) Caffeine Inhibits Direct and Indirect Angiogenesis in Zebrafish Embryos. International Journal of Molecular Sciences. 22(9):.
Abstract
In this study, we report the effects of caffeine on angiogenesis in zebrafish embryos both during normal development and after exposure to Fibroblast Growth Factor 2 (FGF2). As markers of angiogenesis, we measured the length and width of intersegmental vessels (ISVs), performed whole-mount in situ hybridization with fli1 and cadh5 vascular markers, and counted the number of interconnecting vessels (ICVs) in sub-intestinal venous plexus (SIVP). In addition, we measured angiogenesis after performing zebrafish yolk membrane (ZFYM) assay with microinjection of fibroblast growth factor 2 (FGF2) and perivitelline tumor xenograft assay with microinjection of tumorigenic FGF2-overexpressing endothelial (FGF2-T-MAE) cells. The results showed that caffeine treatment causes a shortening and thinning of ISVs along with a decreased expression of the vascular marker genes and a decrease in the number of ICVs in the SIVP. Caffeine was also able to block angiogenesis induced by exogenous FGF2 or FGF2-producing cells. Overall, our results are suggestive of the inhibitory effect of caffeine in both direct and indirect angiogenesis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping