PUBLICATION

Altered non-coding RNA expression profile in F1 progeny 1 year after parental irradiation is linked to adverse effects in zebrafish

Authors
Martín, L., Kamstra, J.H., Hurem, S., Lindeman, L.C., Brede, D.A., Aanes, H., Babiak, I., Arenal, A., Oughton, D., Salbu, B., Lyche, J.L., Aleström, P.
ID
ZDB-PUB-210220-18
Date
2021
Source
Scientific Reports   11: 4142 (Journal)
Registered Authors
Aleström, Peter, Babiak, Igor
Keywords
none
Datasets
GEO:GSE98539, GEO:GSE100470, GEO:GSE152189
MeSH Terms
  • RNA, Untranslated/genetics*
  • DNA Damage/genetics
  • DNA Damage/radiation effects
  • Zebrafish/genetics*
  • Animals
  • Signal Transduction/genetics
  • Signal Transduction/radiation effects
  • Transcriptome/genetics
  • Transcriptome/radiation effects
  • Gamma Rays/adverse effects*
  • DNA Methylation/genetics
  • DNA Methylation/radiation effects
  • Gametogenesis/genetics
  • Gametogenesis/radiation effects
(all 14)
PubMed
33602989 Full text @ Sci. Rep.
Abstract
Gamma radiation produces DNA instability and impaired phenotype. Previously, we observed negative effects on phenotype, DNA methylation, and gene expression profiles, in offspring of zebrafish exposed to gamma radiation during gametogenesis. We hypothesize that previously observed effects are accompanied with changes in the expression profile of non-coding RNAs, inherited by next generations. Non-coding RNA expression profile was analysed in F1 offspring (5.5 h post-fertilization) by high-throughput sequencing 1 year after parental irradiation (8.7 mGy/h, 5.2 Gy total dose). Using our previous F1-γ genome-wide gene expression data (GSE98539), hundreds of mRNAs were predicted as targets of differentially expressed (DE) miRNAs, involved in pathways such as insulin receptor, NFkB and PTEN signalling, linking to apoptosis and cancer. snRNAs belonging to the five major spliceosomal snRNAs were down-regulated in the F1-γ group, Indicating transcriptional and post-transcriptional alterations. In addition, DEpiRNA clusters were associated to 9 transposable elements (TEs) (LTR, LINE, and TIR) (p = 0.0024), probable as a response to the activation of these TEs. Moreover, the expression of the lincRNAs malat-1, and several others was altered in the offspring F1, in concordance with previously observed phenotypical alterations. In conclusion, our results demonstrate diverse gamma radiation-induced alterations in the ncRNA profiles of F1 offspring observable 1 year after parental irradiation.
Genes / Markers
Figures
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Expression
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Phenotype
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Mutations / Transgenics
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Human Disease / Model
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Sequence Targeting Reagents
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Fish
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Antibodies
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Orthology
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Engineered Foreign Genes
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Mapping
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Citation
Martín, L., Kamstra, J.H., Hurem, S., Lindeman, L.C., Brede, D.A., Aanes, H., Babiak, I., Arenal, A., Oughton, D., Salbu, B., Lyche, J.L., Aleström, P. (2021) Altered non-coding RNA expression profile in F1 progeny 1 year after parental irradiation is linked to adverse effects in zebrafish. Scientific Reports. 11:4142.