PUBLICATION
Modulation of the Tissue Expression Pattern of Zebrafish CRP-Like Molecules Suggests a Relevant Antiviral Role in Fish Skin
- Authors
- Bello-Perez, M., Adamek, M., Coll, J., Figueras, A., Novoa, B., Falco, A.
- ID
- ZDB-PUB-210128-15
- Date
- 2021
- Source
- Biology 10(2): (Other)
- Registered Authors
- Figueras, Antonio, Novoa, Beatriz
- Keywords
- SVCV, antiviral, c-reactive protein, mucosal immunity, rag1 mutants, short pentraxins, skin, transcript expression, zebrafish
- MeSH Terms
- none
- PubMed
- 33498981 Full text @ Biology (Basel)
Citation
Bello-Perez, M., Adamek, M., Coll, J., Figueras, A., Novoa, B., Falco, A. (2021) Modulation of the Tissue Expression Pattern of Zebrafish CRP-Like Molecules Suggests a Relevant Antiviral Role in Fish Skin. Biology. 10(2).
Abstract
Recent studies suggest that short pentraxins in fish might serve as biomarkers for not only bacterial infections, as in higher vertebrates including humans, but also for viral ones. These fish orthologs of mammalian short pentraxins are currently attracting interest because of their newly discovered antiviral activity. In the present work, the modulation of the gene expression of all zebrafish short pentraxins (CRP-like proteins, CRP1-7) was extensively analyzed by quantitative polymerase chain reaction. Initially, the tissue distribution of crp1-7 transcripts and how the transcripts varied in response to a bath infection with the spring viremia of carp virus, were determined. The expression of crp1-7 was widely distributed and generally increased after infection (mostly at 5 days post infection), except for crp1 (downregulated). Interestingly, several crp transcription levels significantly increased in skin. Further assays in mutant zebrafish of recombinant activation gene 1 (rag1) showed that all crps (except for crp2, downregulated) were already constitutively highly expressed in skin from rag1 knockouts and only increased moderately after viral infection. Similar results were obtained for most mx isoforms (a reporter gene of the interferon response), suggesting a general overcompensation of the innate immunity in the absence of the adaptive one.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping