Gene expression analysis of crp1–7 in tissues of healthy zebrafish. The expression of crp1–7 was determined by RT-qPCR by using specific primers for each isoform (Table S1). ef1a mRNA was used as the endogenous control to normalize data, which are represented as the mean relative expression level × 10³ ± SD of four different individuals. Statistical differences (p < 0.05, one-way ANOVA) between tissues are represented by: a (different from up to 1–2 tissues), b (different from up to 3–4 tissues), and c (different from up to 5–6 tissues). Data in bar graphs are summarized in a final double gradient colormap (descending blue gradient for values from 0 to 1 and ascending red gradient from values from 1 to ≥750).

Expression modulation of crp1–7 in zebrafish tissues in response to spring viremia of carp virus (SVCV) infection. The transcription levels of crp1–7 and SVCV n in tissues from SVCV-infected zebrafish at 2 and 5 dpi (black and white bars, respectively) were quantified by RT-qPCR. ef1a mRNA was used as the endogenous control in all cases. crp1–7 transcription levels were also normalized to the values obtained from the corresponding samples in non-infected fish. Data are represented as the mean fold changes ± SD for crps and as the mean relative expression level ± SD for SVCV n (four different individuals in all cases). Significant differences were determined by two-way ANOVA and Sidak’s multiple comparison test. Statistical differences between the 2- and 5-dpi groups are represented by keys together with a, b, and c letters on top. Statistical differences between the 2- or 5-dpi groups and the non-infected group are represented by a, b, and c letters just on top of the corresponding bars. a, p ≤ 0.05; b, p ≤ 0.01; c, p ≤ 0.001.

Gene expression analysis of crp1–7 in tissues of healthy zebrafish. The expression of crp1–7 was determined by RT-qPCR by using specific primers for each isoform (Table S1). ef1a mRNA was used as the endogenous control to normalize data, which are represented as the mean relative expression level × 10³ ± SD of four different individuals. Statistical differences (p < 0.05, one-way ANOVA) between tissues are represented by: a (different from up to 1–2 tissues), b (different from up to 3–4 tissues), and c (different from up to 5–6 tissues). Data in bar graphs are summarized in a final double gradient colormap (descending blue gradient for values from 0 to 1 and ascending red gradient from values from 1 to ≥750).