PUBLICATION
Protocol for Disome Profiling to Survey Ribosome Collision in Humans and Zebrafish
- Authors
- Mito, M., Mishima, Y., Iwasaki, S.
- ID
- ZDB-PUB-210101-7
- Date
- 2020
- Source
- STAR protocols 1: 100168 (Other)
- Registered Authors
- Mishima, Yuichiro
- Keywords
- Gene Expression, High Throughput Screening, Sequencing
- MeSH Terms
-
- Animals
- CRISPR-Cas Systems/genetics
- High-Throughput Nucleotide Sequencing/methods*
- Humans
- Polymerase Chain Reaction
- RNA, Guide, Kinetoplastida/metabolism
- RNA, Ribosomal/metabolism
- Ribosomes/metabolism*
- Zebrafish/metabolism*
- PubMed
- 33377062 Full text @ STAR Protoc
Citation
Mito, M., Mishima, Y., Iwasaki, S. (2020) Protocol for Disome Profiling to Survey Ribosome Collision in Humans and Zebrafish. STAR protocols. 1:100168.
Abstract
Ribosomes often encounter obstacles during translation elongation and thus collide with each other. Disome profiling, an optimized ribosome profiling method, specifically sequences the long ribosome footprints generated from collided ribosomes produced by the ribosome pause and thus allows the survey of sites in a genome-wide manner. This protocol details the procedure from lysate preparation of human tissue cultures and zebrafish embryos to sequencing library construction. For complete details on the use and execution of this protocol, please refer to Han et al. (2020).
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping