PUBLICATION

Neuropeptide VF neurons promote sleep via the serotonergic raphe

Authors
Lee, D.A., Oikonomou, G., Cammidge, T., Andreev, A., Hong, Y., Hurley, H., Prober, D.A.
ID
ZDB-PUB-201219-8
Date
2020
Source
eLIFE   9: (Journal)
Registered Authors
Prober, David
Keywords
genetics, neural circuit, neuropeptide VF, neuroscience, raphe, serotonin, sleep, zebrafish
MeSH Terms
  • Animals
  • Hypothalamus/physiology*
  • Neural Pathways/physiology
  • Neurons/metabolism*
  • Neuropeptides/metabolism*
  • Raphe Nuclei/metabolism*
  • Sleep/physiology*
  • Zebrafish
PubMed
33337320 Full text @ Elife
Abstract
Although several sleep-regulating neuronal populations have been identified, little is known about how they interact with each other to control sleep/wake states. We previously identified neuropeptide VF (NPVF) and the hypothalamic neurons that produce it as a sleep-promoting system (Lee et al., 2017). Here we show using zebrafish that npvf-expressing neurons control sleep via the serotonergic raphe nuclei (RN), a hindbrain structure that is critical for sleep in both diurnal zebrafish and nocturnal mice. Using genetic labeling and calcium imaging, we show that npvf-expressing neurons innervate and can activate serotonergic RN neurons. We also demonstrate that chemogenetic or optogenetic stimulation of npvf-expressing neurons induces sleep in a manner that requires NPVF and serotonin in the RN. Finally, we provide genetic evidence that NPVF acts upstream of serotonin in the RN to maintain normal sleep levels. These findings reveal a novel hypothalamic-hindbrain neuronal circuit for sleep/wake control.
Genes / Markers
Figures
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Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes