ZFIN ID: ZDB-PUB-200620-24
Notochordal Signals Establish Phylogenetic Identity of the Teleost Spine
Peskin, B., Henke, K., Cumplido, N., Treaster, S., Harris, M.P., Bagnat, M., Arratia, G.
Date: 2020
Source: Current biology : CB   30(14): 2805-2814.e3 (Journal)
Registered Authors: Bagnat, Michel, Harris, Matthew, Henke, Katrin
Keywords: evolution, extracellular matrix, notochord, segmentation, spine, teleosts, vertebrae, zebrafish
MeSH Terms:
  • Animals
  • Biological Evolution*
  • Body Patterning/genetics*
  • Cellobiose/analogs & derivatives
  • Extracellular Matrix Proteins/genetics
  • Extracellular Matrix Proteins/physiology*
  • Gene Expression Regulation, Developmental/genetics*
  • Gene Expression Regulation, Developmental/physiology*
  • Morphogenesis/genetics*
  • Mutation
  • Notochord/metabolism*
  • Osteoblasts/pathology
  • Phylogeny*
  • Spine/growth & development*
  • Zebrafish/genetics*
  • Zebrafish/growth & development*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/physiology*
PubMed: 32559448 Full text @ Curr. Biol.
The spine is a defining feature of the vertebrate body plan. However, broad differences in vertebral structures and morphogenetic strategies occur across vertebrate groups, clouding the homology between their developmental programs. Analysis of a zebrafish mutant, spondo, whose spine is dysmorphic, prompted us to reconstruct paleontological evidence, highlighting specific transitions during teleost spine evolution. Interestingly, the spondo mutant recapitulates characteristics present in basal fishes, not found in extant teleosts. Further analysis of the mutation implicated the teleost-specific notochord protein, Calymmin, as a key regulator of spine patterning in zebrafish. The mutation in cmn results in loss of notochord sheath segmentation, altering osteoblast migration to the developing spine, and increasing sensitivity to somitogenesis defects associated with congenital scoliosis in amniotes. These data suggest that signals from the notochord define the evolutionary identity of the spine and demonstrate how simple shifts in development can revert traits canalized for about 250 million years.