PUBLICATION

Origin and role of the cerebrospinal fluid bidirectional flow in the central canal

Authors
Thouvenin, O., Keiser, L., Cantaut-Belarif, Y., Carbo-Tano, M., Verweij, F., Jurisch-Yaksi, N., Bardet, P.L., van Niel, G., Gallaire, F., Wyart, C.
ID
ZDB-PUB-200110-11
Date
2020
Source
eLIFE   9: (Journal)
Registered Authors
Bardet, Pierre-Luc, Jurisch-Yaksi, Nathalie, Wyart, Claire
Keywords
physics of living systems, zebrafish
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Biological Transport
  • Cerebral Ventricles/physiology
  • Cerebrospinal Fluid/physiology*
  • Cilia/physiology
  • Embryo, Nonmammalian/physiology
  • Embryonic Development
  • Green Fluorescent Proteins/metabolism
  • Muscle Contraction/physiology
  • Rheology*
  • Spinal Cord/physiology*
  • Zebrafish/embryology
  • Zebrafish/physiology
PubMed
31916933 Full text @ Elife
Abstract
Circulation of the cerebrospinal fluid (CSF) contributes to body axis formation and brain development. Here, we investigated the unexplained origins of the CSF flow bidirectionality in the central canal of the spinal cord of 30 hpf zebrafish embryos and its impact on development. Experiments combined with modeling and simulations demonstrate that the CSF flow is generated locally by caudally-polarized motile cilia along the ventral wall of the central canal. The closed geometry of the canal imposes the average flow rate to be null, explaining the reported bidirectionality. We also demonstrate that at this early stage, motile cilia ensure the proper formation of the central canal. Furthermore, we demonstrate that the bidirectional flow accelerates the transport of particles in the CSF via a coupled convective-diffusive transport process. Our study demonstrates that cilia activity combined with muscle contractions sustain the long-range transport of extracellular lipidic particles, enabling embryonic growth.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes