ZFIN ID: ZDB-PUB-190528-1
Interferon regulatory factor 2 binding protein 2b regulates neutrophil versus macrophage fate during zebrafish definitive myelopoiesis
Wang, L., Gao, S., Wang, H., Xue, C., Liu, X., Yuan, H., Wang, Z., Chen, S., Chen, Z., de Thé, H., Zhang, Y., Zhang, W., Zhu, J., Zhou, J.
Date: 2019
Source: Haematologica   105(2): 325-337 (Journal)
Registered Authors: Chen, Zhu, Zhang, Wenqing, Zhang, Yiyue, Zhu, Jun
Keywords: Granulocytes, Monocytes, Macrophages, Hematopoiesis, Irf2bp2b, NMPs cell fate choice, SUMOylation
MeSH Terms:
  • Animals
  • Carrier Proteins
  • Cell Differentiation
  • DNA-Binding Proteins/genetics*
  • Macrophages
  • Myelopoiesis*
  • Neutrophils
  • Transcription Factors/genetics*
  • Zebrafish*
  • Zebrafish Proteins/genetics*
PubMed: 31123027 Full text @ Haematologica
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ABSTRACT
Proper cell fate choice of neutrophil-macrophage progenitor is essential for adequate myeloid sub-populations during embryonic development and in adulthood. The network governing neutrophil-macrophage progenitor cell fate is composed of several key determinants such as myeloid master regulators CCAAT enhancer binding protein alpha (C/EBPα) and spleen focus forming virus proviral integration oncogene (PU.1). Nevertheless, more regulators remain to be identified and characterized. To ensure balanced commitment of neutrophil-macrophage progenitor toward each lineage, the interplay among these determinants is not only synergistic, but also antagonistic. Depletion of Interferon regulatory factor 2 binding protein 2b (Irf2bp2b), a well known negative transcription regulator, results in a bias in neutrophil-macrophage progenitor cell fate choice which favors macrophages at the expense of neutrophils during the zebrafish definitive myelopoiesis stage in deficient embryos. Mechanistic studies indicate that Interferon regulatory factor 2 binding protein 2b acts as a downstream target of CCAAT enhancer binding protein alpha, to repress spleen focus forming virus proviral integration oncogene expression, and that SUMOylation confers the repressive function of Interferon regulatory factor 2 binding protein 2b. Thus, Interferon regulatory factor 2 binding protein 2b is a novel determinant in the cell fate choice of neutrophil-macrophage progenitor.
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