PUBLICATION
Modulation of DNA Repair Systems in Blind Cavefish during Evolution in Constant Darkness
- Authors
- Zhao, H., Di Mauro, G., Lungu-Mitea, S., Negrini, P., Guarino, A.M., Frigato, E., Braunbeck, T., Ma, H., Lamparter, T., Vallone, D., Bertolucci, C., Foulkes, N.S.
- ID
- ZDB-PUB-181016-4
- Date
- 2018
- Source
- Current biology : CB 28(20): 3229-3243.e4 (Journal)
- Registered Authors
- Bertolucci, Cristiano, Braunbeck, Thomas, Foulkes, Nicholas-Simon, Frigato, Elena, Vallone, Daniela, Zhao, Haiyu
- Keywords
- D-box, DNA repair, UV damage, cavefish, evolution, photolyase, photoreactivation, zebrafish
- MeSH Terms
-
- Animals
- Cyprinidae/growth & development*
- Cyprinidae/physiology
- DNA Repair*
- Darkness
- Evolution, Molecular*
- Fish Proteins/genetics*
- Fish Proteins/metabolism
- Zebrafish/growth & development*
- Zebrafish/physiology
- PubMed
- 30318355 Full text @ Curr. Biol.
Citation
Zhao, H., Di Mauro, G., Lungu-Mitea, S., Negrini, P., Guarino, A.M., Frigato, E., Braunbeck, T., Ma, H., Lamparter, T., Vallone, D., Bertolucci, C., Foulkes, N.S. (2018) Modulation of DNA Repair Systems in Blind Cavefish during Evolution in Constant Darkness. Current biology : CB. 28(20):3229-3243.e4.
Abstract
How the environment shapes the function and evolution of DNA repair systems is poorly understood. In a comparative study using zebrafish and the Somalian blind cavefish, Phreatichthys andruzzii, we reveal that during evolution for millions of years in continuous darkness, photoreactivation DNA repair function has been lost in P. andruzzii. We demonstrate that this loss results in part from loss-of-function mutations in pivotal DNA-repair genes. Specifically, C-terminal truncations in P. andruzzii DASH and 6-4 photolyase render these proteins predominantly cytoplasmic, with consequent loss in their functionality. In addition, we reveal a general absence of light-, UV-, and ROS-induced expression of P. andruzzii DNA-repair genes. This results from a loss of function of the D-box enhancer element, which coordinates and enhances DNA repair in response to sunlight. Our results point to P. andruzzii being the only species described, apart from placental mammals, that lacks the highly evolutionary conserved photoreactivation function. We predict that in the DNA repair systems of P. andruzzii, we may be witnessing the first stages in a process that previously occurred in the ancestors of placental mammals during the Mesozoic era.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping