PUBLICATION

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database

Authors
Pasquier, J., Cabau, C., Nguyen, T., Jouanno, E., Severac, D., Braasch, I., Journot, L., Pontarotti, P., Klopp, C., Postlethwait, J.H., Guiguen, Y., Bobe, J.
ID
ZDB-PUB-181009-10
Date
2016
Source
BMC Genomics   17: 368 (Other)
Registered Authors
Bobe, Julien, Braasch, Ingo, Postlethwait, John H.
Keywords
Assembly, Gar, Gene duplication, Gene expression, Holostean, Mcam, Salmonids, Stra8, Teleosts
MeSH Terms
  • Animals
  • Computational Biology/methods
  • Databases, Nucleic Acid*
  • Evolution, Molecular*
  • Fishes/classification
  • Fishes/genetics*
  • Gene Duplication*
  • Gene Expression*
  • Gene Expression Profiling
  • Genome*
  • Phylogeny
  • Transcriptome
  • Web Browser
PubMed
27189481 Full text @ BMC Genomics
Abstract
With more than 30,000 species, ray-finned fish represent approximately half of vertebrates. The evolution of ray-finned fish was impacted by several whole genome duplication (WGD) events including a teleost-specific WGD event (TGD) that occurred at the root of the teleost lineage about 350 million years ago (Mya) and more recent WGD events in salmonids, carps, suckers and others. In plants and animals, WGD events are associated with adaptive radiations and evolutionary innovations. WGD-spurred innovation may be especially relevant in the case of teleost fish, which colonized a wide diversity of habitats on earth, including many extreme environments. Fish biodiversity, the use of fish models for human medicine and ecological studies, and the importance of fish in human nutrition, fuel an important need for the characterization of gene expression repertoires and corresponding evolutionary histories of ray-finned fish genes. To this aim, we performed transcriptome analyses and developed the PhyloFish database to provide (i) de novo assembled gene repertoires in 23 different ray-finned fish species including two holosteans (i.e. a group that diverged from teleosts before TGD) and 21 teleosts (including six salmonids), and (ii) gene expression levels in ten different tissues and organs (and embryos for many) in the same species. This resource was generated using a common deep RNA sequencing protocol to obtain the most exhaustive gene repertoire possible in each species that allows between-species comparisons to study the evolution of gene expression in different lineages. The PhyloFish database described here can be accessed and searched using RNAbrowse, a simple and efficient solution to give access to RNA-seq de novo assembled transcripts.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping