ZFIN ID: ZDB-PUB-180201-3
Tbx5a lineage tracing shows cardiomyocyte plasticity during zebrafish heart regeneration
Sánchez-Iranzo, H., Galardi-Castilla, M., Minguillón, C., Sanz-Morejón, A., González-Rosa, J.M., Felker, A., Ernst, A., Guzmán-Martínez, G., Mosimann, C., Mercader, N.
Date: 2018
Source: Nature communications   9: 428 (Journal)
Registered Authors: Felker, Anastasia, Gonzalez-Rosa, Juan Manuel, Mercader Huber, Nadia, Minguillón, Carolina, Mosimann, Christian, Sánchez Iranzo, Héctor
Keywords: none
Microarrays: GEO:GSE87596
MeSH Terms:
  • Animals
  • Animals, Genetically Modified
  • Cell Differentiation
  • Cell Lineage/genetics
  • Cell Tracking
  • Embryo, Nonmammalian
  • Gene Expression Regulation, Developmental
  • Genes, Reporter
  • Green Fluorescent Proteins/genetics
  • Green Fluorescent Proteins/metabolism
  • Heart Ventricles/cytology*
  • Heart Ventricles/growth & development
  • Heart Ventricles/metabolism
  • Luminescent Proteins/genetics
  • Luminescent Proteins/metabolism
  • Myocardium/cytology*
  • Myocardium/metabolism
  • Myocytes, Cardiac/cytology*
  • Myocytes, Cardiac/metabolism
  • Myosin Light Chains/genetics
  • Myosin Light Chains/metabolism
  • Organogenesis/genetics
  • Regeneration/genetics*
  • Stem Cells/cytology
  • Stem Cells/metabolism
  • T-Box Domain Proteins/deficiency
  • T-Box Domain Proteins/genetics*
  • Zebrafish/genetics*
  • Zebrafish/growth & development
  • Zebrafish/metabolism
PubMed: 29382818 Full text @ Nat. Commun.
During development, mesodermal progenitors from the first heart field (FHF) form a primitive cardiac tube, to which progenitors from the second heart field (SHF) are added. The contribution of FHF and SHF progenitors to the adult zebrafish heart has not been studied to date. Here we find, using genetic tbx5a lineage tracing tools, that the ventricular myocardium in the adult zebrafish is mainly derived from tbx5a+ cells, with a small contribution from tbx5a- SHF progenitors. Notably, ablation of ventricular tbx5a+-derived cardiomyocytes in the embryo is compensated by expansion of SHF-derived cells. In the adult, tbx5a expression is restricted to the trabeculae and excluded from the outer cortical layer. tbx5a-lineage tracing revealed that trabecular cardiomyocytes can switch their fate and differentiate into cortical myocardium during adult heart regeneration. We conclude that a high degree of cardiomyocyte cell fate plasticity contributes to efficient regeneration.