ZFIN ID: ZDB-PUB-170928-21
A vertebrate-specific and essential role for osterix in osteogenesis revealed by gene knockout in the teleost medaka
Yu, T., Graf, M., Renn, J., Schartl, M., Larionova, D., Huysseune, A., Witten, P.E., Winkler, C.
Date: 2017
Source: Development (Cambridge, England)   144: 265-271 (Journal)
Registered Authors: Graf, Martin, Huysseune, Ann, Renn, Joerg, Schartl, Manfred, Winkler, Christoph, Witten, P. Eckhard
Keywords: Bone modelling, Medaka, Osteoblasts, Osteogenesis, Skeleton
MeSH Terms:
  • Animals
  • Animals, Genetically Modified
  • Calcification, Physiologic/genetics
  • Gene Expression Regulation, Developmental
  • Gene Knockout Techniques
  • Notochord/embryology
  • Oryzias/embryology*
  • Oryzias/genetics*
  • Osteogenesis/genetics*
  • Phylogeny
  • Species Specificity
  • Transcription Factors/genetics
  • Transcription Factors/physiology*
  • Vertebrates/embryology
  • Vertebrates/genetics
  • Zebrafish Proteins/physiology
PubMed: 27993982 Full text @ Development
osterix (osx; sp7) encodes a zinc-finger transcription factor that controls osteoblast differentiation in mammals. Although identified in all vertebrate lineages, its role in non-mammalian bone formation remains elusive. Here, we show that an osx mutation in medaka results in severe bone defects and larval lethality. Pre-osteoblasts fail to differentiate leading to severe intramembranous and perichondral ossification defects. The notochord sheath mineralizes normally, supporting the idea of an osteoblast-independent mechanism for teleost vertebral centra formation. This study establishes a key role for Osx for bone formation in a non-mammalian species, and reveals conserved and non-conserved features in vertebrate bone formation.