ZFIN ID: ZDB-PERS-970324-1
Huysseune, Ann
Email: Ann.Huysseune@UGent.be
Affiliation: Vertebrate Morphology and Developmental Biology Lab (VMDB)
Address: Department of Biology Ghent University K.-L. Ledeganckstraat 35 Ghent, B-9000 Belgium
Country: Belgium
Phone: 32-9-264-5229
Fax: 32-9-264-5344


Cotti, S., Huysseune, A., Larionova, D., Koppe, W., Forlino, A., Witten, P.E. (2022) Compression Fractures and Partial Phenotype Rescue With a Low Phosphorus Diet in the Chihuahua Zebrafish Osteogenesis Imperfecta Model. Frontiers in endocrinology. 13:851879
Huysseune, A., Soenens, M., Sire, J.Y., Witten, P.E. (2022) High-Resolution Histology for Craniofacial Studies on Zebrafish and Other Teleost Models. Methods in molecular biology (Clifton, N.J.). 2403:249-262
Huysseune, A., Cerny, R., Witten, P.E. (2021) The conundrum of pharyngeal teeth origin: the role of germ layers, pouches, and gill slits. Biological reviews of the Cambridge Philosophical Society. 97(1):414-447
Rosa, J.T., Witten, P.E., Huysseune, A. (2021) Cells at the Edge: The Dentin-Bone Interface in Zebrafish Teeth. Frontiers in Physiology. 12:723210
Qu, M., Liu, Y., Zhang, Y., Wan, S., Ravi, V., Qin, G., Jiang, H., Wang, X., Zhang, H., Zhang, B., Gao, Z., Huysseune, A., Zhang, Z., Zhang, H., Chen, Z., Yu, H., Wu, Y., Tang, L., Li, C., Zhong, J., Ma, L., Wang, F., Zheng, H., Yin, J., Witten, P.E., Meyer, A., Venkatesh, B., Lin, Q. (2021) Seadragon genome analysis provides insights into its phenotype and sex determination locus. Science advances. 7(34)
Kotzé, S.H., Huysseune, A. (2020) Mucin histochemistry as a tool to assess rostral digestive tract health in a teleost model (Danio rerio). Journal of fish diseases. 43(12):1603-1606
Cotti, S., Huysseune, A., Koppe, W., Rücklin, M., Marone, F., Wölfel, E.M., Fiedler, I.A.K., Busse, B., Forlino, A., Witten, P.E. (2020) More Bone with Less Minerals? The Effects of Dietary Phosphorus on the Post-Cranial Skeleton in Zebrafish. International Journal of Molecular Sciences. 21(15):
Oralová, V., Rosa, J.T., Larionova, D., Witten, P.E., Huysseune, A. (2020) Multiple epithelia are required to develop teeth deep inside the pharynx. Proceedings of the National Academy of Sciences of the United States of America. 117(21):11503-11512
Bek, J.W., De Clercq, A., De Saffel, H., Soenens, M., Huysseune, A., Witten, P.E., Coucke, P., Willaert, A. (2020) Photoconvertible fluorescent proteins: A versatile tool in zebrafish skeletal imaging. Journal of Fish Biology. 98(4):1007-1017
Teixeira Rosa, J., Oralová, V., Larionova, D., Eisenhoffer, G.T., Eckhard Witten, P., Huysseune, A. (2019) Periderm invasion contributes to epithelial formation in the teleost pharynx. Scientific Reports. 9:10082
Oralová, V., Rosa, J.T., Soenens, M., Bek, J.W., Willaert, A., Witten, P.E., Huysseune, A. (2019) Beyond the whole-mount phenotype: high-resolution imaging in fluorescence-based applications on zebrafish. Biology Open. 8(5):
De Clercq, A., Perrott, M.R., Davie, P.S., Preece, M.A., Owen, M.A.G., Huysseune, A., Witten, P.E. (2018) Temperature sensitive regions of the Chinook salmon vertebral column: Vestiges and meristic variation. Journal of morphology. 279(9):1301-1311
Xu, P., Balczerski, B., Ciozda, A., Louie, K., Oralova, V., Huysseune, A., Crump, J.G. (2018) Fox proteins are modular competency factors for facial cartilage and tooth specification. Development (Cambridge, England). 145(12)
Woltmann,I., Shkil, F., De Clercq, A., Huysseune, A., and Witten, P. E. (2018) Supernumerary teeth in the pharyngeal dentition of slow‐developing zebrafish (Danio rerio, Hamilton, 1822). Zeitschrift fur angewandte Ichthyologie = Journal of applied ichthyology.
Suniaga, S., Rolvien, T., Vom Scheidt, A., Fiedler, I.A.K., Bale, H.A., Huysseune, A., Witten, P.E., Amling, M., Busse, B. (2018) Increased mechanical loading through controlled swimming exercise induces bone formation and mineralization in adult zebrafish. Scientific Reports. 8:3646
Kague, E., Witten, P.E., Soenens, M., Campos, C.L., Lubiana, T., Fisher, S., Hammond, C., Brown, K.R., Passos-Bueno, M.R., Huysseune, A. (2018) Zebrafish sp7 mutants show tooth cycling independent of attachment, eruption and poor differentiation of teeth. Developmental Biology. 435(2):176-184
De Clercq, A., Perrott, M.R., Davie, P.S., Preece, M.A., Huysseune, A., Witten, P.E. (2017) The external phenotype-skeleton link in post-hatch farmed Chinook salmon (Oncorhynchus tshawytscha). Journal of fish diseases. 41(3):511-527
De Clercq, A., Perrott, M.R., Davie, P.S., Preece, M.A., Wybourne, B., Ruff, N., Huysseune, A., Witten, P.E. (2017) Vertebral column regionalisation in Chinook salmon, Oncorhynchus tshawytscha. Journal of anatomy. 231(4):500-514
Tan, W.H., Witten, P.E., Winkler, C., Au, D.W.T., Huysseune, A. (2017) Telomerase Expression in Medaka ( Oryzias melastigma) Pharyngeal Teeth. Journal of dental research. 96:678-684
Yu, T., Graf, M., Renn, J., Schartl, M., Larionova, D., Huysseune, A., Witten, P.E., Winkler, C. (2017) A vertebrate-specific and essential role for osterix in osteogenesis revealed by gene knockout in the teleost medaka. Development (Cambridge, England). 144:265-271
Witten, P.E., Harris, M.P., Huysseune, A., Winkler, C. (2017) Small teleost fish provide new insights into human skeletal diseases. Methods in cell biology. 138:321-346
Gistelinck, C., Eckhard Witten, P., Huysseune, A., Symoens, S., Malfait, F., Larionova, D., Simoens, P., Dierick, M., Van Hoorebeke, L., De Paepe, A., Kwon, R.Y., Weis, M., Eyre, D.R., Willaert, A., Coucke, P.J. (2016) Loss of Type I Collagen Telopeptide Lysyl Hydroxylation Causes Musculoskeletal Abnormalities in a Zebrafish Model of Bruck Syndrome. Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research. 31(11):1930-1942
Verstraeten, B., van Hengel, J., Huysseune, A. (2016) Beta-Catenin and Plakoglobin Expression during Zebrafish Tooth Development and Replacement. PLoS One. 11:e0148114
Gistelinck, C., Gioia, R., Gagliardi, A., Tonelli, F., Marchese, L., Bianchi, L., Landi, C., Bini, L., Huysseune, A., Witten, P.E., Staes, A., Gevaert, K., De Rocker, N., Menten, B., Malfait, F., Leikin, S., Carra, S., Tenni, R., Rossi, A., De Paepe, A., Coucke, P., Willaert, A., Forlino, A. (2016) Zebrafish Collagen Type I: Molecular and Biochemical Characterization of the Major Structural Protein in Bone and Skin. Scientific Reports. 6:21540
Bensimon-Brito, A., Cardeira, J., Dionísio, G., Huysseune, A., Cancela, M.L., Witten, P.E. (2016) Revisiting in vivo staining with alizarin red S - a valuable approach to analyse zebrafish skeletal mineralization during development and regeneration. BMC Developmental Biology. 16:2
Symoens, S., Barnes, A.M., Gistelinck, C., Malfait, F., Guillemyn, B., Steyaert, W., Syx, D., D'hondt, S., Biervliet, M., De Backer, J., Witten, E.P., Leikin, S., Makareeva, E., Gillessen-Kaesbach, G., Huysseune, A., Vleminckx, K., Willaert, A., De Paepe, A., Marini, J.C., Coucke, P.J. (2015) Genetic Defects in TAPT1 Disrupt Ciliogenesis and Cause a Complex Lethal Osteochondrodysplasia. American journal of human genetics. 97(4):521-34
Crucke, J., Van de Kelft, A., Huysseune, A. (2015) The innervation of the zebrafish pharyngeal jaws and teeth. Journal of anatomy. 227(1):62-71
Willems, B., Tao, S., Yu, T., Huysseune, A., Witten, P.E., Winkler, C. (2015) The Wnt Co-Receptor Lrp5 Is Required for Cranial Neural Crest Cell Migration in Zebrafish. PLoS One. 10:e0131768
Bruneel, B., Mathä, M., Paesen, R., Ameloot, M., Weninger, W.J., Huysseune, A. (2015) Imaging the Zebrafish Dentition: From Traditional Approaches to Emerging Technologies. Zebrafish. 12(1):1-10
Crucke, J., Huysseune, A. (2015) Blocking VEGF Signaling Delays Development of Replacement Teeth in Zebrafish. Journal of dental research. 94(1):157-65
Huysseune, A., Soenens, M., Elderweirdt, F. (2014) Wnt signaling during tooth replacement in zebrafish (Danio rerio): pitfalls and perspectives. Frontiers in Physiology. 5:386
Crucke, J., and Huysseune, A. (2013) Unravelling the blood supply to the zebrafish pharyngeal jaws and teeth. Journal of anatomy. 223(4):399-409
Hosen, M.J., Vanakker, O.M., Willaert, A., Huysseune, A., Coucke, P., and De Paepe, A. (2013) Zebrafish models for ectopic mineralization disorders: practical issues from morpholino design to post-injection observations. Frontiers in Epigenomics. 4:74
Verstraeten, B., van Hengel, J., Sanders, E., Van Roy, F., and Huysseune, A. (2013) N-cadherin is Required for Cytodifferentiation during Zebrafish Odontogenesis. Journal of dental research. 92(4):365-370
Bensimon-Brito, A., Cardeira, J., Cancela, L., Huysseune, A., and Witten, E. (2012) Distinct patterns of notochord mineralization in zebrafish coincide with the localization of Osteocalcin isoform 1 during early vertebral centra formation. BMC Developmental Biology. 12(1):28
Bensimon-Brito, A., Cancela, M.L., Huysseune, A., and Witten, P.E. (2012) Vestiges, rudiments and fusion events: the zebrafish caudal fin endoskeleton in an evo-devo perspective. Evolution & development. 14(1):116-127
Verstraeten, B., Sanders, E., and Huysseune, A. (2012) Whole mount immunohistochemistry and in situ hybridization of larval and adult zebrafish dental tissues. Methods in molecular biology (Clifton, N.J.). 887:179-191
Dewit, J., Witten, P.E., and Huysseune, A. (2011) The mechanism of cartilage subdivision in the reorganization of the zebrafish pectoral fin endoskeleton. Journal of experimental zoology. Part B, Molecular and developmental evolution. 316(8):584-97
Verstraeten, B., Sanders, E., van Hengel, J., and Huysseune, A. (2010) Zebrafish teeth as a model for repetitive epithelial morphogenesis: dynamics of E-cadherin expression. BMC Developmental Biology. 10:58
Dewit, J., Witten, P. E., Willems, M., Huysseune, A. (2010) The distribution of fibronectin in developing zebrafish (Danio rerio) cartilage. Zeitschrift fur angewandte Ichthyologie = Journal of applied ichthyology. 26(2):205-209
Bensimon-Brito, A., Cancela, M. L., Huysseune, A., Witten, P. E. (2010) The zebrafish (Danio rerio) caudal complex – a model to study vertebral body fusion. Zeitschrift fur angewandte Ichthyologie = Journal of applied ichthyology. 26(2):235-238
Witten, P.E., and Huysseune, A. (2009) A comparative view on mechanisms and functions of skeletal remodelling in teleost fish, with special emphasis on osteoclasts and their function. Biological reviews of the Cambridge Philosophical Society. 84(2):315-346
Verreijdt, L., Debiais-Thibaud, M., Borday-Birraux, V., Van der Heyden, C., Sire, J.Y., and Huysseune, A. (2006) Expression of the dlx gene family during formation of the cranial bones in the zebrafish (Danio rerio): Differential involvement in the visceral skeleton and braincase. Developmental dynamics : an official publication of the American Association of Anatomists. 235(5):1371-1389
Borday-Birraux, V., Van der Heyden, C., Debiais-Thibaud, M., Verreijdt, L., Stock, D.W., Huysseune, A., and Sire, J.Y. (2006) Expression of Dlx genes during the development of the zebrafish pharyngeal dentition: evolutionary implications. Evolution & development. 8(2):130-141
Huysseune, A. (2006) Formation of a successional dental lamina in the zebrafish (Danio rerio): support for a local control of replacement tooth initiation. The International journal of developmental biology. 50(7):637-643
Van der Heyden, C., Allizard, F., Sire, J.Y., and Huysseune, A. (2005) Tooth development in vitro in two teleost fish, the cichlid Hemichromis bimaculatus and the cyprinid Danio rerio. Cell and tissue research. 321(3):375-389
Laurenti, P., Thaëron, C., Allizard, F., Huysseune, A., and Sire, J.Y. (2004) Cellular expression of eve1 suggests its requirement for the differentiation of the ameloblasts and for the initiation and morphogenesis of the first tooth in the zebrafish (Danio rerio). Developmental dynamics : an official publication of the American Association of Anatomists. 230(4):727-733
Huysseune, A., and Thesleff, I. (2004) Continuous tooth replacement: the possible involvement of epithelial stem cells. BioEssays : news and reviews in molecular, cellular and developmental biology. 26(6):665-671
Huysseune, A. and Sire, J.-Y. (2004) The role of epithelial remodelling in tooth eruption in larval zebrafish. Cell and tissue research. 315(1):85-95
Sire, J.-Y., Davit-Beal, T., Delgado, S., van der Heyden, C., and Huysseune, A. (2002) First-generation teeth in nonmammalian lineages: evidence for a conserved ancestral character?. Microscopy research and technique. 59:408-434
Huysseune, A., van der Heyden, C., Verreijdt, L., Wautier, K., and van Damme, N. (2002) Fish dentitions as paradigms for odontogenic questions. Connective tissue research. 43(2-3):98-102
Verreijdt, L., Vandervennet, E., Sire, J.Y., and Huysseune, A. (2002) Developmental differences between cranial bones in the zebrafish (Danio rerio): Some preliminary light and TEM observations. Connective tissue research. 43(2-3):109-112
van der Heyden, C., Wautier, K., and Huysseune, A. (2001) Tooth succession in the zebrafish (Danio rerio). Archives of oral biology. 46(11):1051-1058
Wautier, K., van der Heyden, and Huysseune, A. (2001) Changes in pharyngeal tooth form during successive replacements in the zebrafish. Journal of morphology. 248(3):298
Wautier, K., van der Heyden, C., and Huysseune, A. (2001) A quantitative analysis of pharyngeal tooth shape in the zebrafish (Danio rerio, Teleostei, Cyprinidae). Archives of oral biology. 46(1):67-75
van der Heyden, C. and Huysseune, A. (2000) Dynamics of tooth formation and replacement in the zebrafish (Danio rerio) (Teleostei, Cyprinidae). Developmental dynamics : an official publication of the American Association of Anatomists. 219(4):486-496
van der Heyden, C., Huysseune, A., and Sire, J.-Y. (2000) Development and fine structure of pharyngeal replacement teeth in juvenile zebrafish (Danio rerio)(Teleostei, Cyprinidae). Cell and tissue research. 302:205-219
Huysseune, A., van der Heyden, C., and Sire, J.Y. (1998) Early development of the zebrafish (Danio rerio) pharyngeal dentition (Teleostei, Cyprinidae). Anatomy and embryology. 198:289-305
Miyake, T., Huysseune, A., Hall, B.K., and Smith, M.M. (1991) Development of a CAM-grafting system for skeletal tissues of fishes. American zoologist. 31:47A