PUBLICATION

Dietary lipid content reorganizes gut microbiota and probiotic L. rhamnosus attenuates obesity and enhances catabolic hormonal milieu in zebrafish

Authors
Falcinelli, S., Rodiles, A., Hatef, A., Picchietti, S., Cossignani, L., Merrifield, D.L., Unniappan, S., Carnevali, O.
ID
ZDB-PUB-170720-10
Date
2017
Source
Scientific Reports   7: 5512 (Journal)
Registered Authors
Carnevali, Oliana, Unniappan, Suraj
Keywords
Molecular biology, Transcription
MeSH Terms
  • Animals
  • Appetite/drug effects
  • Calcium-Binding Proteins/genetics
  • Calcium-Binding Proteins/metabolism
  • Cholesterol/metabolism
  • DNA-Binding Proteins/genetics
  • DNA-Binding Proteins/metabolism
  • Diet, High-Fat
  • Dietary Fats*
  • Female
  • Gastrointestinal Microbiome/drug effects*
  • Intestines/pathology
  • Intestines/ultrastructure
  • Lacticaseibacillus rhamnosus/physiology*
  • Lipid Droplets/metabolism
  • Lipid Droplets/ultrastructure
  • Male
  • Nerve Tissue Proteins/genetics
  • Nerve Tissue Proteins/metabolism
  • Obesity/prevention & control*
  • Obesity/veterinary
  • Principal Component Analysis
  • Probiotics/pharmacology*
  • Probiotics/therapeutic use
  • Triglycerides/metabolism
  • Zebrafish/metabolism*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
PubMed
28717234 Full text @ Sci. Rep.
Abstract
In the present study, we explored whether dietary lipid content influences the gut microbiome in adult zebrafish. Diets containing three different lipid levels (high [HFD], medium [MFD], and low [LFD]) were administered with or without the supplementation of Lactobacillus rhamnosus (P) to zebrafish in order to explore how the dietary lipid content may influence the gut microbiome. Dietary lipid content shifted the gut microbiome structure. The addition of L. rhamnosus in the diets, induced transcriptional reduction of orexigenic genes, upregulation of anorexigenic genes, and transcriptional decrease of genes involved in cholesterol and triglyceride (TAG) metabolism, concomitantly with lower content of cholesterol and TAG. Probiotic feeding also decreased nesfatin-1 peptide in HFD-P and attenuated weight gain in HFD-P and MFD-P fed zebrafish, but not in LFD-P group. Intestinal ultrastructure was not affected by dietary fat level or probiotic inclusion. In conclusion, these findings underline the role of fat content in the diet in altering gut microbiota community by shifting phylotype composition and highlight the potential of probiotics to attenuate high-fat diet-related metabolic disorder.
Genes / Markers
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Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping